Reports on the visualization of calcium-dependent protein-protein interactions in livings cells by fluorescence readouts using a circularly permuted green fluorescent protein (GFP). Imaging of HeLa cells expressing pericams; Monitoring of protein heterodimerization.Nagai...
Folding of the green fluorescent protein (GFP) from Aequorea victoria is characterized by autocatalytic formation of its p-hydroxybenzylideneimidazolidone chromophore, which is located in the center of an 11-stranded β-barrel. We have analyzed the in vivo folding of 20 circularly permuted variants...
Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP 喜欢 0 阅读量: 41 作者:Otilia,Zapata-HommerOliver,Griesbeck 摘要: Background The green fluorescent protein (GFP) has been widely used in cell biology as a marker of gene expression, label of cellular structures, ...
-dependent protein–protein interactions in livingcellsbyfluorescencereadouts,weusedacircularlypermutedgreenfluorescent protein (cpGFP), in which the amino and carboxylportions had been interchanged and reconnected by a short spacerbetween the original termini. The cpGFP was fused to calmodulinand its t...
We introduced new termini on surface loops of green fluorescent protein (GFP) by linking the original ones. The mechanical properties of two circularly permuted GFPs (cpGFPs) were examined by atomic force microscopy (AFM) and compared with those of the base GFPs. The unfolding results revealed...
We investigated the mechanical unfolding of single circularly permuted green fluorescent protein (cpGFP) with atomic force microscopy (AFM). The molecule was stretched from its N- and C-termini by an external force causing an elongation of the polypeptide chain up to its full length. The features...
Upon light irradiation, cpLOV2 alters the conformation of AcrIIA4 to diminish its dCas9-inhibitory activity, thereby restoring the function of dCas9-VPR to turn on GFP expression. (right) Quantification of light-inducible changes in GFP expression (n = 3 independent biological replicates; ...
of this method is first demonstrated with insertions of circularly permuted GFP into maltose-binding protein, coupled with fluorescence-activated cell sorting (FACS), to isolate biosensor proteins that are allosterically regulated by maltose. ... DC Nadler 被引量: 0发表: 2014年 Design rules for pr...
dynamics with betterspatial and temporal resolution than before. Our study provides animportantguideforthedevelopmentandimprovementofindicatorsusing GFP-based FRET.Cameleons are genetically encoded fluorescent indicators forCa 2? based o
2007. Engineering a circularly permuted GFP scaffold for peptide presentation. J. Mol. Recognit. 20:367-378. http: //dx.doi.org/10.1002/jmr.844.Matthias Paschke,Christian Tiede,Wolfgang Ho"hne.Engineering a circularly permuted GFP scaffold for peptide presentation. Journal of Molecular Recognition...