An expression vector for the expression of a desired gene in an animal cell is described, which contains a chicken beta -actin gene promoter or a derivative thereof and downstream thereof a restriction enzyme site for the incorporation of a desired gene. Furthermore corresponding recombinant ...
Smirnova MG, Kiselev SL, Gnuchev NV, Birchall JP, Pearson JP (2002) Role of the pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6 and interleukin-8 in the pathogenesis of the otitis media with effusion. Eur Cytokine Netw 13:161–172 CAS PubMed Google...
The activity of the CMV and β-actin promoters was downregulated during selection of stable transfectants and during differentiation to the Flk1 stage, while the CMV immediate enhancer/β-actin promoter in the pCAGIPuro-GFP vector led to 100% of stably transfected undifferentiated and differentiated...
In most cases of in ovo electroporation, the ubiquitously active promoter CAGGS has been used, which contains the CMV and chicken beta-actin promoter (Niwa et al., 1991). This promoter becomes active soon after electroporated into cells (Momose et al., 1999). During development, it is ...
We detected the contribution of DE proteins to IFN-β promoter activity induced by chicken derived MDA5. Results showed that several DE proteins could significantly modulate IFN-β promoter activity induced by MDA5 when compared with that of pCAGGS-HA as control. Some of these proteins, such as...
Kawakami (NIG) was digested with BglII-XhoI to remove the cassette containing EF1 promoter-EGFP-SV40polyA29. This site was blunt-ended, and inserted with the fragment of pT2K-BI-TRE-gapEGFP, which contains bidirectional tetracycline-responsive element (TRE) with two minimal promoters of CMV in...
CMV enhancer + chicken beta-actin promoter + rabbit beta-globin intron; eGFP, enhanced green fluorescent protein; pA, bovine growth hormone polyadenylation signal; cMAR, chicken lysozyme matrix attachment region (BamHI-PvuI fragment); cHS4, the core region of chicken beta-globin insulator (250bp)...
DOUBLE-STRANDED/SELF-COMPLEMENTARY VECTORS WITH A TRUNCATED CBA PROMOTER AND METHODS OF GENE DELIVERY The present inventors concerns vectors carrying a truncated chimeric CMV-chicken 尾-actin (smCBA) promoter in which the hybrid chicken 尾-actin/rabbit 尾-globin intron is greatly shortened, and their...
bmc cell biology the cmv early enhancer/chicken β actin (cag) promoter can be used to drive transgene expression during the differentiation of murine embryonic stem cells into vascular progenitors 来自 dx.doi.org 喜欢 0 阅读量: 25 作者: AN Alexopoulou,JR Couchman,JR Whiteford 展开 ...
The CC genotype is predicted to bind to the Adf-1 transcription factor, which can enhance the promoter activity of SERPINB6 and promote its expression. Adf-1 has also been reported to be a key modulator of the dynamic organization of the actin cytoskeleton [56]. Further validation of the ...