已有研究报道cfRNA用于一些实体肿瘤,包括乳腺癌、结直肠癌、滤泡性淋巴瘤和肝细胞癌等的早期诊断。因此,cfRNA被认为是用于早期检测生物标志物的分析物来源。 今天跟大家分享一篇发表于cancers(IF=6.126)上的一篇利用cell-free RNA (cfRNA)测序检测早期肿瘤的文章Plasma Next Generation Sequencing and Droplet Digital-q...
使用SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian Kit制备 total RNA-Seq 文库,并在 Illumina NovaSeq 6000 测序(PE 200),每个样本获得约 1×109 reads。 参考文献:Paul ED, et al. Multiplexed RNA-FISH-guided Laser Capture Microdissection RNA Sequencing Improves Breast Cancer Molecular S...
使用SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian Kit制备total RNA-Seq文库,并在Illumina NovaSeq 6000测序(PE 200),每个样本获得约1×109reads。 参考文献:Paul ED, et al. Multiplexed RNA-FISH-guided Laser Capture Microdissection RNA Sequencing Improves Breast Cancer Molecular Subtyping, ...
Cell-free RNA (cfRNA) from liquid biopsies combined with high-thought sequencing technology, named cfRNA-Seq, has been broadly used to detect biomarkers in the fields of cancer diagnosis, fetal abnormality detection, neurodegenerative disorder investigation and pathogen detection. However, the developmen...
路胜cfDNA+cfRNA联检LiquidHALLMARK®的融合检测性能验证 摘要编号：#3040 标题：A cell-free RNA-based next-generation sequencing (NGS) assay for the detection of actionable gene fusions in patients with non–small cell lung cancer (NSCLC).背景 大部分的液体活检使用血浆游离DNA（cfDNA）分析突变，但是...
Using targeted sequencing, we explored the potential of this approach to detect mutations, fusion mRNA, and copy number variation (CNV) in solid tumors and hematologic neoplasms.Methods:Peripheral blood cfTNA and cfRNA were extracted from B-cell lymphoid neoplasms (#105), T-cell neoplasms (#16...
1. Xi Yang, Taifu Wang, Sujun Zhu, et al. PALM-Seq: integrated sequencing of cell-free long RNA and small RNA[J]. bioRxiv, 2019: 686055. 2. Penghui Yang, Yingze Zhao, Jie Li, Chuanyu Liu, Linnan Zhu, Jie...
该研究使用华大研究院自主开发的PALM-Seq技术 [1, PALM-Seq: integrated sequencing of cell-free long RNA and small RNA],一次实验即可对不同症状新冠患者及健康对照血浆中的多种游离RNA进行全谱检测(包括 游离mRNA、lncRNA、miRNA及tRNA 等,移除了 rRNA)。共鉴定出了7个可明显区分新冠患者与健康对照的血浆...
8.Whole genome bisulfite sequencing, WGBS-Seq 常见的ctDNA 检测技术的对比 ctDNA 检测技术的对比 五、ctDNA优势 1.与蛋白类标记物相比,ctDNA 检测很少出现假阳性,因为 ctDNA 来自肿瘤细胞基因组突变。 2. ctDNA 半衰期短,能准确反映肿瘤当前情况。剑桥大学和约翰霍普金斯大学合作团队研究表明,检测乳腺癌和肠癌时...
该研究使用华大研究院自主开发的PALM-Seq技术 [1, PALM-Seq: integrated sequencing of cell-free long RNA and small RNA],一次实验即可对不同症状新冠患者及健康对照血浆中的多种游离RNA进行全谱检测(包括 游离mRNA、lncRNA、miRNA及tRNA 等,移除了 rRNA)。共鉴定出了7个可明显区分新冠患者与健康对照的血浆...