Sites that fold into G4 structures in vitro (OQs) are highlighted in purple for the plus and minus strands. The orange box highlights the regions of a close-up view. c, Venn diagrams illustrating the overlap of
a BMDC from WT or Irg1−/− mice were HDM-pulsed (100 μg/ ml) or sham-pulsed with PBS for 16 h and were adoptively transferred to naïve WT mice (0.2 × 106 cells in 40 μl sterile saline; i.n. administration). Recipient WT mice received HDM challenges (50 ...
After 4 hours, cells were stained with 5 μg/mL propidium iodide (Sigma Aldrich) and analyzed by flow cytometry. Specific lysis was calculated as the percentage of propidium iodide+ cells among total Green Tracker+ cells, minus the background level (as determined by target cells incubated ...
Data are representative of two independent experiments. All data are represented as mean ± s.e.m. FMO, Fluorescence Minus One Control. Source data Full size image Next, we investigated the impact of TCR avidity on T cell activation in the TME. tdLNs and tumors were collected from ...
After lentiviral packaging with HEK293T cells, cells were infected with lentiviruses and selected by incubation with 2 μg/mL puromycin for 3 days. For transfection of RNASEH1 D210N mutant construct, cells were transfected with ppyCAG_RNASEH1_D210N_V5 plasmid (Addgene, MA, USA, 111904) ...
Cells were washed with the wash buffer A and then incubated with Probe Anti-Mouse PLUS (Cat# DUO92001) and Probe Anti-Rabbit MINUS (Cat# DUO92005; Sigma-Aldrich) for 1 h at 37°C. Coverslips were washed with buffer A and then incubated with a DNA ligase diluted in Ligation buffer for...
The tumour cells were cultured as a monolayer in RPMI 1640 medium supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 2 mmol/L glutamine, 1 mmol/L sodium pyruvate, 10% heat-inactivated fetal calf serum (all products from Invitrogen-GIBCO, Germany). The cells were routinely ...
Briefly, a total of 1 × 104 cells were placed in 30 μl of USSC growth mediumACF (plus or minus bFGF), or in stem cell proliferation medium without fetal calf serum (plus or minus bFGF), and dropped onto the inside of a Petri dish lid. Drops were incubated upside down in a ...
Dark gray: 5 h minus 3 h, medium gray: 24 h minus 3 h, light gray: 48 h minus 3 h. ATP hydrolysis by MOVAS cells after 48 h (Figure 4A, black trace) was evidenced by a decrease in the 1231 cm−1 band corresponding to vibrational mode of phosphate belonging to ATP [39,40,...
The occupancy level of Brg-1 or Sp1 at the SPARC promoter is represented as the ratio of signal from IP samples versus that of the input minus background of IgG control. The relative occupancy level of Brg-1 or Sp1 in 4T1 cells is set as 1. Data represented as mean ± SEM (n = ...