Data are representative of two independent experiments. All data are represented as mean ± s.e.m. FMO, Fluorescence Minus One Control. Source data Full size image Next, we investigated the impact of TCR avidity on T cell activation in the TME. tdLNs and tumors were collected from ...
Low-quality cells were filtered if the library size or the number of expressed genes (counts larger than 0) was smaller than predefined thresholds. Both thresholds were defined as the medians of all cells minus 3× the median absolute deviation. Furthermore, if the proportion of mitochondrial g...
In short, the cells were co-stained over night at 4 °C using the following antibodies: SETD1B (Abcam, Cambridge, UK; #ab113984; 1:1000) and ZEB1 (R&D Systems, Wiesbaden, Germany; #639914; 10 µg/ml). The next day, the PLA probe solution was added to the cells as ...
After lentiviral packaging with HEK293T cells, cells were infected with lentiviruses and selected by incubation with 2 μg/mL puromycin for 3 days. For transfection of RNASEH1 D210N mutant construct, cells were transfected with ppyCAG_RNASEH1_D210N_V5 plasmid (Addgene, MA, USA, 111904) ...
A method of obtaining expanded and activated natural killer (NK) cells with the phenotype CD3−CD56+ and NK-like T cells with the phenotype CD3+CD56+ comprises providing a cell sample of peripheral blood from a tumor bearing subject; isolating cells from the blood sample and re-suspending...
Cells were subcultured at 1:5 every 2–3 days on 150-mm tissue culture dishes treated with 0.1% gelatin. For differentiation, 129/SvEv mouse ES cells were plated on bacteriological plates in ES culture medium minus LIF as described in Aiba et al. (3). Medium was replaced on days 2, ...
Insulin-secreting INS1E cells were incubated in the absence or presence of 5 μm S1P for 24 h and thereafter. A, caspase-3 activation was measured by the Caspase3-Glo assay after a 24-h incubation with 600 units/ml IL-1β or a cytokine mixture (60 units/ml IL-1β, 185 units/ml...
Durand B, Fero ML, Roberts JM, Raff MC: p27Kip1 alters the response of cells to mitogen and is part of a cell-intrinsic timer that arrests the cell cycle and initiates differentiation. Curr Biol. 1998, 8: 431-440. 10.1016/S0960-9822(98)70177-0. Article CAS PubMed Google Scholar ...
Briefly, a total of 1 × 104 cells were placed in 30 μl of USSC growth mediumACF (plus or minus bFGF), or in stem cell proliferation medium without fetal calf serum (plus or minus bFGF), and dropped onto the inside of a Petri dish lid. Drops were incubated upside down in a ...
i IL-17A, IL-13 and IFNγ levels in supernatants obtained from Tem cell co-cultures with WT or Il1r−/−CD11c + splenic DCs in the presence of αCD3 (30 ng ml−1) after 48 h of stimulation. Control in receptor staining refers to fluorescence minus one control. IL-...