Immunoprecipitation was performed using an anti-HA antibody or with rabbit IgG (Cell Signaling Technology) as a negative control. PCR products were resolved by electrophoresis in a 2% Agarose-gel (Invitrogen). Primer sequences for ChIP assay are provided in Supplementary Data 6. Single-cell RNA ...
Biogenesis of ApoExos has been reported to be associated with S1P signaling initiated by S1PR1/3 from the plasma membrane at the early apoptotic phase, progressing to endosomal maturation, mainly by the downstream Gβγ subunit of S1PR1/3 and subsequent actin mobilization52. Despite the limited ...
For instance, live cell imaging populations over some days have proved that signaling dynamics can manage cell reactions. Therefore, having computational tools can increase the efficiency of cellular analysis with exact results in order to ease a deeper understanding the connection of the changes of ...
The JAK/STAT signaling pathway is a critical player in cancer treatment and multi-target therapy, with abnormal activation observed in various solid malignancies such as breast, lung, liver, head and neck, and stomach cancers [65]. This heightened JAK/STAT signaling has been associated with poore...
This oxidative damage can lead to mutations in critical genes, alterations in signaling pathways, and impaired cellular functions. In the context of cancer, the accumulation of ROS-induced DNA damage can contribute to genetic instability and the development of malignant tumors [3]. Additionally, ...
If insufficient oxygen permeates to all cells within the niche, this can adversely impact cell health, resulting in a cascade of unintentional stress signaling by dying cells, negatively influencing the niche microenvironment. These considerations are important for nutrients such as oxygen, glucose, ...
Surprisingly, ABC transporter proteins such as multi-drug resistance protein-1, leading to therapy-resistance of CSCs is controlled by Hh signaling [92]. Therefore, ABC transporter proteins can be used as a surface markers for CSC identification, and their ability to transport drugs itself enables...
Alternatively, cells can be classified into pre-determined phenotype classes using a supervised approach, and the profile is then defined as the fraction of cells in each phenotype class9. However, many cell phenotypes are better considered as a continuum of varying morphologies rather than discrete...
Cell Communication and Signaling volume 21, Article number: 191 (2023) Cite this article 4206 Accesses 1 Altmetric Metrics details Abstract In recent years, increasingly more non-coding RNAs have been detected with the development of high-throughput sequencing technology, including microRNAs (miRNAs),...
The illumination of their complex life cycle, which includes receptor biosynthesis, membrane targeting, ligand binding, signaling, internalization, recycling and degradation, will provide new insights into the relationship between spatial receptor distribution and function. This review covers the existing ...