CD4+CD25+ Tregs (2.5x105) were co-cultured either with CD11b+Gr-1+ MDSCs or with CD11b+ cells at a ratio of 1:1 in the absence or presence of IL-6 (40ng/ml) for 48 hours. IL-10 knockdown (KD) CD11b+Gr-1+ MDSCs were generated by IL-10 siRNA (10nM) transfection for 6 ...
越来越多 的证据表明一 群具有Gr~1 和C I〕 l l b标 志 的髓 系来 源抑 制性 细胞( 卿 e l o id 一 e i rv eds 叩 p r e s s o re e ll s,M DSC )在荷瘤小鼠的脾 脏、骨髓、淋巴结、肿瘤 原发部 位和浸润组 织中 大量 聚积,与肿 瘤进展 关系十分 密切,有关 MD S C与 肿...
Easily and efficiently isolate highly purified mouse myeloid-derived suppressor cells (MDSCs) (CD11b+Gr1+) from mouse splenocytes, bone marrow, or peripheral blood samples by immunomagnetic negative selection, with the EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit. Widely used in published ...
(67.2 ±8.7)mg,P <0.01],and the proportion of splenic Gr-1+ CD11b +MDSCsin EAE mice significantly increased (P <0.01 vs controls). Consistent with the results of immunofluorescent staining,a significantlyincreased proportion of Gr-1+ CD11b +MDSCs in spinal cords of EAE mice was observed...
源的抑制 细胞 (Gr-1 CDllb myeloid—derived suppressor cells,MDSC) 。进一步研究证实,该群细胞具有很强 的免疫抑制作用 ,参与调节疾病 的进展及严重程度 , 但在MS 中对该群细胞的研究却鲜有报道。基于此 , 我们设计了本项实验 ,旨在探讨Gr.1 CD1lb MDSC 在MS 动物模型.实验性 自身 免疫性脑脊...
suppressor cells，MDSCs)是骨髓来源的一群异质性细胞，是树突状细胞(dendritic cells，DCs)、巨噬细胞和(或)粒细胞的前体，具有显著抑制免疫细胞应答的能力。小鼠MDSCs的表型为CD11b+Gr-1+，分两个亚型:粒细胞样的MDSCs(CD11b+Ly6C-Ly6G+)和单核细胞样的MDSCs(CD11b+Ly6C+Ly6G-)。
(t=5.06,P=0.007);采用CD11b磁珠从注射脂多糖的小 鼠脾脏中分离出的CD11b+ Gr-1+ MDSCs纯度高达84.0%±4.2%:MTT法显示在MDSCs共培养后脾脏T细胞增殖水平明显低于对照组,且此作用与MDSCs的数量呈正相关 (F=46.26,P=0.000).结论 CD11b磁珠可分离提取出纯度较高的脂多糖来源的MDSCs,且脂多糖诱导的MDSCs对...
通过流式细胞术分析(图2A),与健康组相比,4T1对照组肺和血液中CD11b+Gr-1+细胞的比例至少增加了3倍,脾脏中CD11b+Gr-1+细胞的比例增加了20倍(图2B-D)。4T1-空腹组的CD3e+CD4+T细胞比例甚至高于健康组(图2B和D)。此外,还通...
Studies have shown that bone marrow-derived cells play an important role in tumor recurrence after chemotherapy and radiotherapy. In this study, we examined the relationship between the accumulation of Gr-1+CD11b+ cells and tumor recurrence after irradia