cell lysis buffer (20 mmol/L Tris-HCT, pH 7.5, 150 mmol/L NaCl, 1 mmol/L Na2EDTA, 1 mmol/L EGTA, 1% Triton, 2.5 mmol/L sodium pyrophosphate, 1 mmol/L Na3VO4, 1 µg/mL leupeptin, 1 mmol/L phenylmethylsulfonyl fluoride) (Cell Signaling Technology, Inc., Danvers, MA, USA)....