Cancer-Finder算法在大规模数据集上的应用潜力 在对Cancer Single-cell Expression Map (CancerSCEM)数据库的超过500 000个细胞进行注释时,Cancer-Finder算法展现了其在大规模数据集上的应用潜力。它在一小时内就能完成快速预测,并且与数...
此外，Cancer-Finder在多个数据集上的表现均优于现有的其他工具，如SCEVAN、CopyKAT、CaSee和ikarus等。在对超过500 000个细胞的Cancer Single-cell Expression Map (CancerSCEM)数据库进行注释时，Cancer-Finder在一小时内就能快速预测，且与数据库提供的恶性细胞百分比高度相关。Cancer-Finder算法的高准确率识别能力 该...
A screen of interactions between genes involved in the cell’s response to DNA damage has revealed several previously unreported ‘synthetic lethalities’, in which disrupting a pair of genes, but not either gene alone, causes cell death. The resulting map of genetic interactions could help to ...
spatial transcriptomics; Xenium In Situ; cancer evolution; cancer-associated fibroblast; extracellular matrix remodeling; SPP1+ macrophage; regulatory T cell; immune escape; NOTCH1 Introduction Para_01 理解致癌过程中复杂的机制对于探索肿瘤进化和识别早期干预的目标至关重要。 最近基因组学和单细胞转录组学的...
Code used for the assignment of cells to reference expression profiles is available at https://github.com/broadinstitute/single_cell_classification. References McGranahan, N. & Swanton, C. Biological and therapeutic impact of intratumor heterogeneity in cancer evolution. Cancer Cell 27, 15–26 (...
(D) Dot plot showing the expression levels of marker genes in each cell type. (E) The bar chart showed the proportion of 13 GBCs in each cell type. (F) Heatmap displaying the relative enrichment of each cell type in cancer and adjacent normal tissues of 13 GBCs. AC, adenocarcinoma; ...
在可获得数据的5例患者中,对治疗有反应的3名患者经曲美替尼治疗后,MAP2K1和MAP2K2激酶活性降低;而在2例无反应的患者中,MAP2K1活性没有变化,MAPK26活性增加。此外,还观察到无反应患者中与细胞周期相关的几种激酶活性增加,包括CDK6、CDK9、WEE1和AURKB。
Heatmap of the mean expression of all markers in the myeloid panel, calculated across CD45+ leukocytes (value range 0-1 post-transformation/normalization). To visualize every single immune population isolated from the different brain tumor samples, we created a two-dimensional graph using the dimen...
2)RTK-RAS-MAPK通路激活:RTK-RAS-MAPK通路中的激活突变也很常见,导致尽管抑制了突变KRAS,RAS-MAPK信号仍重新激活。除KRAS本身的改变外,BRAF、MAP2K1和NRAS是该通路中最常突变的基因。KRAS上游的MAPK通路改变较为罕见,但已有NF1功能丧失突变的报道。 3)PI3K通路激活:除了上游RTK激活PI3K通路外,PI3K通路本身的改变也...
Single-cell systems pharmacology identifies development-driven drug response and combination therapy in B cell acute lymphoblastic leukemia Long-lasting mRNA-encoded interleukin-2 restores CD8 ARID1A orchestrates SWI/SNF-mediated sequential binding of transcription factors with ARID1A loss driving pre-memory...