Caco-2 cell monolayers model has been extensively utilized assays for permeability assessment and transport characteristics of compounds in both pharmaceutical industries and academia.5–7)Derived from human colon adenocarcinoma cells, Caco-2 cells could spontaneously differentiate into small intestinal microvi...
我们首先通过严谨的实验步骤，如Cellular Uptake and Transport Characteristics of FL118 Derivatives in Caco-2 Cell Monolayers研究，验证了模型的精确性和重现性。Caco-2细胞单层模型因其高度模拟了小肠微绒毛上皮细胞的特性，成为了药物吸收与转运研究的黄金标准（5–7）。细胞分化后，它们能够稳定表达与...
【5】Tavelin S , Johan Gråsjö, Taipalensuu J , et al. Applications of Epithelial Cell Culture in Studies of Drug Transport[J]. Methods in Molecular Biology, 2002, 188:233-272. 【6】Hidalgo I J , Raub T J , Borchardt R T...
[Key words]Caco-2 cell monolayer model;isorhapontigenin;transport mechanism 異丹叶大黄素(isorhapontigenin,ISO)存在于买麻藤类植物中,具有良好的抗炎[1-2],抗氧化[3-5],治疗胰岛素抵抗及肥胖[6]等活性。近年來,有研究发现其有显著的抗膀胱癌活性[7-11]。有研究表明,小鼠灌胃不同浓度的异丹叶大黄素,...
Utilization and Protection,Guangzhou Institute ofGeochemistry,Chinese Academy of Sciences,Guangzhou 510640,China)Abstract The Caco-2 cell monolayer,isolated from human colon carcinoma,has been wildly used intoxicology study and used as a tool to study the intestinal uptake and transport processes of ...
Fig 4Effect ofPEG600on transport of OS across Caco-2 cell monolayer(n = 3) 3.8 P-gp抑制剂verapamil对OS在Caco-2细胞模型中转运的影响通过考察HBSS配制成含10μM verapamil的15μM OS溶液在Caco-2细胞模型中转运情况,结果表明,加入P-gp抑制剂verapamil后,与不加P-gp抑制剂verapamil的15μM OS溶液相比...
1. 1 镉目前对镉的转运与吸收研究比较广泛,镉在 Caco-2 细胞系主要通过主动运输进行跨细胞转运(transcellular transport)[4],细胞内镉结合位点的饱和限制了镉的进一步转运[5,6]。对于镉的细胞摄取存在特定饱和机制,可敏感地被汞所抑制[7]。氯化镉、谷胱甘肽镉和植物螯合物镉等不同形态的镉在 Caco-2 细胞中的...
[13] 陈江华,杨林,邓中平.山豆根中主要成分在Caco-2细胞模型上的转运特性[J].中国实验方剂学杂志,2015,21(13):5-8. [14] Chen Y,Liu X,Pan R,et al.Intestinal transport of 3,6′-disinap-oylsucrose, a major active component of Polygala tenuifolia, using Caco-2 cell monolayer and in situ ...
结论 HCFU 在Caco-2细胞中以主动和被动2种形式吸收，其吸收存在外排机制，MRP2可能对HCFU 的吸收有外排作用。 关键词：卡莫氟；Caco-2细胞模型；吸收；转运 中图分类号：R333.3；R966 文献标识码：A 文章编号：1001-2494(2009)21-1637-06 Uptake and Transport Characteristics of Carmofur in Caco...
Caco-2, PAMPA和MDCK等工具常用于测定化合物体外渗透性,评估化合物的吸收性质,筛选优势化合物进入体内实验研究。在研究过程中,常见于某些化合物渗透性试验中回收率较低,因此本文就两个问题展开阐述:1、造成回收率较低的原因;2、化合物回收率的高低对试验结果的影响。就以往的研究经验而言,造成回收率较低的原因可能...