In addition, mitochondrial calcium imaging showed that mitochondrial Ca2+ flux was significantly reduced in PRKN-mutant patient dopaminergic neurons compared to the control. These results suggest a defect in calcium flux from ER to mitochondria is due to the decreased ERMCS in PRKN-mutant patient ...
D Time delay between a specific Ca2+ flux and other ferroptotic events (unrelated Ca2+, change in cell rounding and PI) observed upon Erastin-1 treatment. E Graphical representation of the sequence of events observed during Erastin-1-induced ferroptosis. F Kinetics of increase of the normalized...
Ca2+ signaling is not a single event but the result of orchestrated spatiotemporal changes in Ca2+ flux from different cellular and extracellular compartments [3] acting in concert with other signaling pathways [4]. In lymphocytes, the key event following the engagement of a T-cell receptor (...
A comparison of the time course of BDNF transcription (measured by the run-on assay) and the time course of mature BDNF mRNA transcription (Northern and PCR assays) revealed that the induction of BDNF transcription occurs in parallel with an increase in the mature BDNF mRNA. However, the ...
(Dai et al.2022; Camunas-Soler et al.2020). Functional heterogeneity in islet cells has been long recognized, with variations in β cell insulin release, electrophysiological activity and Ca2+flux (Rorsman et al.2012; Pipeleers1992; Salomon and Meda1986). In parallel to this, single-cell ...
Interestingly, decreases or increases in the intracellular pH shut down Ca2+ flux through the BI-1 channel but not in the pore-dead D213A mutant [7]). These observations are supported by a recent study characterizing the three-dimensional structure and pH-sensitive Ca2+-flux properties of a ...
The expression of proinflammatory mediators was determined by reverse transcriptase polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). Results We demonstrated that an intracellular Ca2+ flux in HCECs was triggered by A. fumigatus hyphae and could be reduced by pre-...
We show that PAR2 is expressed in PSMC and that PAR2 activation mediates a biphasic elevation in intracellular Ca and phosphorylation of myosin light chain 20 (MLC20), causing cellular contraction as assessed in a gel contraction assay. Intracellular Ca flux was inhibited by a phosphoinositide ...
Mitochondrial Ca2+ uniporter contributed to this flux, which favored ATP production and cell migration. In conclusion, this study reveals a novel molecular mechanism controlled by BclxL to promote cancer cell migration and supports the use of BH3 mimetics as therapeutic options not only to kill ...
In- terestingly, the Mn2+ quenching assay, which is widely employed to monitor basal Ca2+ entry in non-excitable cells [70,75,101], revealed that SOCE was constitutively activated in C-hMSCs and was significantly enhanced in ACM C-hMSCs (Figure 4C,D) [4]. Constitutive SOCE regulates ...