Roles of Ca2+ and protein kinase C (PKC) in the regulation of acid/base transport in isolated rabbit proximal tubules were investigated by measuring cytosolic Ca2+ concentrations ([Ca2+]i) and cell pH (pHi) with fluorescent probes. Ionomycin (0.2 microM) increased [Ca2+]i by approximately 20...
Investigates the role of calcium and protein kinase C (PKC) in the regulation of acid/base transport in isolated proximal tubules. Effects of ionomycin on intracelular calcium concentration, sodium/bicarbonate cotransporter and sodium-hydrogen exchanger; Intracelular pH response to sodium reduction with...
One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. They are, therefore...
展开 关键词: Amino Acid Sequence Animals Baculoviridae Base Sequence Carrier Proteins Cloning, Molecular DNA, Complementary Genetic Vectors Humans Insects DOI: 10.1111/j.1748-1716.1994.tb09762.x 被引量: 20 年份: 1994 收藏 引用 批量引用 报错 分享 全部...
展开 关键词: Calcium Escherichia coli Nucleotidases Oryza sativa Adenosine Phosphosulfate Amino Acid Sequence Base Sequence Binding Sites Genes, Bacterial Magnesium DOI: 10.1074/jbc.270.49.29105 被引量: 143 年份: 1995 收藏 引用 批量引用 报错 分享 全部...
Probing the role of negatively charged amino acid residues in ion permeation of skeletal muscle ryanodine receptor. Biophys. J. 89, 256–265 (2005). Article PubMed PubMed Central CAS Google Scholar Xu, L., Wang, Y., Gillespie, D. & Meissner, G. Two rings of negative charges in the...
分类号:密级:学校代码:10165学号:201011039逢宰师耗大学硕士学位论文M92+/Ca2+与碱基和水相互作用的理论研究作者姓名:***业:研究方向:导师姓名:***化学理论与计算化学宫利东教授2013年4月 学位论文独创性声明本人承诺:所呈交的学位论文是本人在导师指导下所取得的研究成果。论文中除特别加以标注和致谢的地方外,不包...
Each lobe is composed of a pair of calcium-binding EF-hands consisting of 29 amino acid residues. The affinity of the C-terminal lobe for calcium ion is higher than that of the N-terminal lobe (Mahling et al., 2021). CaM as adaptor protein is thought to lack enzymatic activity on its...
The intracellular solutions contained (in mM): "0 Ca2+", 100 K-aspartate, 15 KCl, 10 EGTA, 10 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), or Scientific Reports | 5:15509 | DOI: 10.1038/srep15509 7 www.nature.com/scientificreports/ e"w1xi ttμrh MacNeClalOua2lHa...
(B) Effects of bumetanide (Bum, 500 mol/L, basolateral) and the endoplasmic reticulum (ER) Ca2ϩ-ATPase inhibitor cyclopiazonic acid (CPA, 50 mol/L, both sides) on UTP-mediated peak responses. UTP-induced Isc after pretreatment with bumetanide or CPA is shown as percentage of UTP...