In the maximum intensity projection of the MRP2 channel, the background was subtracted using the Rolling Ball algorithm, and the intensity histogram was stretched and equalized. We applied a manual threshold at an intensity level of 50 to binarize the image. Afterward, we performed morphological ...
Following an algorithm based on liver expression and the number of sequences per candidate to further filter the list (see "Methods"), 47 candidate genes were selected for validation (Supplementary Table 1). Among them was HNF4A, a gene encoding a transcription factor previously known to ...