Evaluation of the C. DIFFICILE TOXIN A/B IITM, C. DIFF CHEK-60TM, and PREMIER TOXIN A&BTM with the cytotoxin assay for the detection of Clostridium difficile GDH antigen and toxins A and B in feces - International Journal of Infectious Diseases...
Laboratory tests include detection of C.difficile common antigen (i.e., glutamate dehydrogenase), cell culture tests for Toxin A and/or Toxin B, EIA for Toxin A and /or B, bacterial culture and PCR (1). We evaluated a new screening test, the C.DIFF CHEKTM, in two formats, the C....
DIFF QUIK CHEK COMPLETE® test (Alere North America, Inc.). Samples were considered positive if both GDH and toxin antigens were detected. Those that were inconclusive by this method (GDH antigen positive, toxin negative) were reflexed to a molecular detection method which evaluated the ...
All C. difficile isolates were typed by PCR ribotyping, and the isolates were classified as toxigenic or non-toxigenic based on the empirical knowledge of the association between toxin-positivity and ribotype. The overall sensitivity, specificity, and positive and negative predictive values were ...
Vimentin staining of tubular cross-section was assessed in 50 consecutive fields ( Â 250) using 0, no positive cells; 1 þ , few (1 or 2) positive tubular cells in the tubule; 2 þ , 42, but less than half of cells stained; 3 þ , more than half of cells stained in the...
Diff Quik Chek -TechLab, Blacksburg VA; (b) BD GeneOhm Cdiff assay, BD Diagnostics, San Diego, CA; (c) Portrait Toxigenic C. difficile Assay, Great Basin, West Valley City, UT, stool toxin A/B (EIA) Tox A/B Quick Check from January 2010 to May 2010; C difficile toxin B PCR ...
Erratum to: Role of fecal Clostridium difficile load in discrepancies between toxin tests and PCR: is quantitation the next step in C. difficile testing? Eur J Clin Microbiol Infect Dis. 2012;31:3301.Leslie JL, Cohen SH, Solnick JV, Polage CR. Role of fecal Clostridium difficile load in ...
Specific GPI anchored proteins, such as carcinoembryonic antigen and mesothelin, have been described as potential biomarkers for certain cancers; however, the overall levels of GPI anchored proteins present in plasma from cases of human cancers have not been evaluated. We have developed the use of ...
“Last year a study was published that turned this on its head and appeared to prove that Clostridium difficilestrainsproducing Toxin A alone were innocuous, and that only Toxin B was essential for disease. This had significant implications — already we have seen a move within the diagnostics i...
A false negative, on the other hand, occurs when the person tests negative, suggesting they are healthy, when they actually do have the disease. To draw a ROC curve, the true positive rate (TPR) and false positive rate (FPR) are determined as the decision threshold is varied continuously....