This study evaluated the performance of three commercial NAATs (M10, Xpert, and BD MAX assays) using 200 archived stool specimens initially tested as glutamate dehydrogenase (GDH)-positive but toxin-negative by GDH/toxin A/B enzyme immunoassay, the first step in the two-step algorithm. All ...
as another toxin, called binary toxin, whose role in C. difficle-associated disease is not known.) Mortality rates in Quebec’s hospitals quadrupled and C. difficle-associated disease incidence were five times higher than the historic incidence. The most important risk factor for the C. diff...
Among 107 PCR-positive samples, 46 (43.0 %) had toxins detected by immunoassay and an additional 18 (16.8 %) had toxin detected by the cytotoxin assay yielding 64 (59.8 %) toxin-positive and 43 (40.2 %) toxin-negative samples. Overall, toxin-negative samples with C. difficile had 101–...
The C.DIFF CHEKTM is an enzyme immunoassay for use as a screening test to detect C. difficile antigen, glutamate dehydogenase, in fecal samples. The goals of this evaluation were to determine the sensitivity, specificity, positive predictive value, negative predictive value and correlation of ...
Fidaxomicin (Fdx) is widely used to treat Clostridioides difficile (Cdiff) infections, but the molecular basis of its narrow-spectrum activity in the human gut microbiome remains unknown. Cdiff infections are a leading cause of nosocomial deaths1. Fidaxo
All samples were confirmed negative for the binary toxin gene ctdB and 11% of the isolates were positive for ctdA gene. Interestingly, one isolate harbored the binary toxin gene (cdtA+) and tested negative for both toxins A and B. We believe that combining the standard culture method with ...
Toxin B is essential for virulence of Clostridium difficile. Nature 458, 1176–1179 (2009). Article CAS PubMed PubMed Central ADS Google Scholar Drudy, D., Fanning, S. & Kyne, L. Toxin A-negative, toxin B-positive Clostridium difficile. Int J. Infect. Dis. 11, 5–10 (2007). ...
All C. difficile isolates were typed by PCR ribotyping, and the isolates were classified as toxigenic or non-toxigenic based on the empirical knowledge of the association between toxin-positivity and ribotype. The overall sensitivity, specificity, and positive and negative predictive values were ...
(Fig.1a). Another group of mice were given the antibiotic but were not challenged withC. difficile(noC. diff, or uninfected mice). Mutation oftcdRhas been reported to significantly reduce levels of both toxin gene expression and toxin protein production in vitro34,35. Nearly five-fold fewer...
“Our research, which has recreated that original experiment, but resulted in a different outcome —we were able to show in the laboratory that the mutated Clostridium difficile strain in which produced only Toxin A was deadly.” Clostridium difficileinfectionis the most significant cause of hospital...