This study evaluated the performance of three commercial NAATs (M10, Xpert, and BD MAX assays) using 200 archived stool specimens initially tested as glutamate dehydrogenase (GDH)-positive but toxin-negative by GDH/toxin A/B enzyme immunoassay, the first step in the two-step algorithm. All ...
The C.DIFF CHEKTM is an enzyme immunoassay for use as a screening test to detect C. difficile antigen, glutamate dehydogenase, in fecal samples. The goals of this evaluation were to determine the sensitivity, specificity, positive predictive value, negative predictive value and correlation of ...
DIFF QUIK CHEK COMPLETE® test (Alere North America, Inc.). Samples were considered positive if both GDH and toxin antigens were detected. Those that were inconclusive by this method (GDH antigen positive, toxin negative) were reflexed to a molecular detection method which evaluated the ...
associated with CDI recurrence was the use of enzyme immunoassay (EIA) testing vs. PCR for initial CDI diagnosis (p= 0.046). This finding is consistent with analyses of the parent study, and may be due to higher-false positive rates or detection of non-toxin elaboratingC. difficilestrains wi...
Positive GDH or toxin results corresponded to lower PCR cycle threshold values (P 0.05). The application of the 2-step algorithm should be thoroughly evaluated in immunocompromised patient populations before implementation. (C) 2018 Elsevier Inc. All rights reserved.Ashraf, ZuhhaRahmati, ElhamBender...
METHODS. In October 2022, our system began reflex testing all PCR-positive stool samples with the C. DIFF QUIK CHEK COMPLETE (Techlab), an enzyme immunoassay–based test with results for the glutamate dehydrogenase antigen (GDH) and C difficile toxin A/B. Hospital-onset (HO) CDI and CDI ...
doi:10.1016/j.diagmicrobio.2018.12.010Zuhha AshrafElham RahmatiJeffrey M. BenderNeha NandaRosemary C. SheElsevier
DIFF QUIK CHEK COMPLETE (QCC; TechLab), which detects glutamate dehydrogenase (GDH) antigen (QCC-Ag) and toxin A/B (QCC-Tox) simultaneously, and compared it to the laboratory diagnostics for CDI currently in use in a tertiary hospital setting with a high prevalence of CDI. QCC, RIDASCREEN...
Of 150 PCR-positive specimens, 52 (34.7%) were toxin-positive and 126 (84.0%) were GDH positive. Positive GDH or toxin results corresponded to lower PCR cycle threshold values (P?P?=?0.05) and diarrhea (91.0% vs. 79.4%, P?P?=?0.004) or laxative use (24.6% vs 36.1%, P?=?0.02)...
diff toxins A/B with a multistep algorithm using a GDH-and-toxin EIA and toxins A and B arbitrated by a semiquantitative CCNA [112]. Upon evaluation, the researchers reported that Clarity had 96.2% negative agreement with GDH−/toxin− samples, 100% positive agreement with GDH+/toxin+ ...