BMC Genomics (2018) 19:305 Page 3 of 11 TruSeqTM RNA Sample Preparation Kit (Illumina, San Diego, California, USA), and paired end reads of 100 bp were sequenced using the Illumina® HiSeq 2000 plat- form. These procedures were performed by Macrogen (South Korea). Cleaning and ...
After blocking in 5% non-fat milk for 2 h, the membranes were incubated with primary antibodies: FUT4 (1:1000, Proteintech, Wuhan, China), CD44 (1:1000, Abcam, Cambridge, UK), LTL (1:2000, Vector, California, USA), LeY (1:500, Abcam), p-GSK3β (1:500, Affinity Biosciences, ...
Division of Biology, MC 156-29, California Institute of Technology, Pasadena, 91125, California, USA Ali Mortazavi, Brian A Williams, Kenneth McCue, Lorian Schaeffer & Barbara Wold Corresponding author Correspondence to Barbara Wold. Supplementary information Supplementary Text and Figures Supplementary Fi...
Sutton, et al., “TIGR Assembler: A New Tool for Assembling Large Shotgun Sequencing Projects,” Genome Science & Tech., 1:9-19 (1995). Svinarchuk, et al., “Inhibition of HIV proliferation in MT-4 cells by antisense oligonucleotide conjugated to lipophilic groups” Biochimie 75:49-54 ...
Finally, we examined the role of MeCP2 in dendritic growth and spine development in hippocampal slice culture preparations. We used gene gun-based DNA delivery on rat postnatal day 7 (P7) hippocampal slice cultures and found that introduction of WT MeCP2 significantly decreased dendritic growth,...
(1:2000, Vector, California, USA), LeY (1:500, Abcam), p-GSK3β(1:500, Affinity Biosciences, Cincinnati, OH, USA), GSK3β(1:500, Affinity Biosciences),β-catenin (1:500, Affinity Biosciences), PCNA (1:100, Proteintech) and GAPDH (1:5000, Proteintech) at 4 °C overnight. ...
Prior to sequencing, RNA quality was measured for each sample using an Agilent 2100 Bioanalyzer®. One library per sample was prepared using the Illumina® TruSeqTM RNA Sample Preparation Kit (Illumina, San Diego, California, USA), and paired end reads of 100 bp were sequenced using the ...