At the end of IEF, gel is extruded from the tube and equilibrated in buffer containing SDS to prepare the proteins for migration into the second dimension SDS gel. The tube gel is placed on top of a polyacrylamide gel contain...
Proteins are separated in SDS-PAGE and quantified using densitometry. On an average 19.3 bands from fresh and 15.6 bands from RNAlater storage cells were obtained with a molecular weight ranging from 25 to > 250 kDa. RNAlater storage generated a fewer number and lesser q...
Additional Information Licensing Caveats: Sold under license from The Regents of the University of California. Product Datasheets COA view current batch SDS Reconstitution Calculator Molarity Calculator Reconstitution Calculator The reconstitution calculator allows you to quickly calculate the volume of a reagen...
Protein samples were used for SDS-PAGE followed by western blotting. Nitrocellulose membranes were stained with primary antibodies against COX4I1 (1 : 500), MFN2 (1.500), TUBB (1 : 1000), PGC-1α (1 : 500), TRX1 (1 : 1000), TOMM20 (1 : 1000), TFAM (1 : 1000), SOD2 (1 ...
The T-PER reagent utilizes a non-denaturing detergent in 25 mM bicine, 150 mM NaCl (pH 7.6), and is used in conjunction with mechanical or manual homogenization. Furthermore, T-PER reagent was validated for protein extraction from fresh and ...
run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies used....
Supplier Page Compare Product + See More (10) View All (29) ScienCell Research Laboratories Enzyme-free Cell Dissociation Solution, 100 ml Description: ECDS is used to detach adherent primary cells from a culture surface. It is for research use only. Not for use in ... ...
Cell type used was lung fibroblasts for (a–d) and cardiac fibroblasts for (e). Full size image mTORC1 activation stimulates translation41, cytoplasmic targeting of many different macromolecules, and macromolecular crowding42. We therefore asked whether we could detect circadian oscillations in the ...
Tris-glycine-SDS (TG-SDS) buffer is used for SDS polyacrylamide gel electrophoresis (SDS-PAGE) of protein. These products are powders for preparing Tris-glycine-SDS buffer.
Tris-acetate-EDTA (TAE) buffer is used for agarose and polyacrylamide gel electrophoresis of nucleic acid. 1 L of 50X TAE buffer can be easily prepared by dissolving the powder in water.