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The BrdU incorporation is more evident using the indirect method. A.3.2 Anticipated results It is recommanded to perform each experiment using a negative and a positive control sample. The negative sample, in order to have a correct setting of instrument, is assessed following all the steps ...
25、r, both direct and indirect immunofluorescence can be used.TheBrdU incorporation is more evident using the indirect method.A.3.2 Anticipated resultsIt is recommanded to perform each experiment using a negative andapositive control sample. The negative sample, in order to have acorrectsetting of...
'Cell-substratum interactions trigger key signaling pathways that modulate growth control and tissue-specific gene expression. We have previously shown that abolishing adhesive interactions by suspension culture results in G(0) arrest of myoblasts. We report that blocking intracellular transmission of adhesi...
Dot blot analysis to compare BrdU incorporation by MCM depleted and control cells.Isabelle, CrevelGilles, CrevelThierry, GostanChristelle, de RentyVincent, CoulonSue, Cotterill
Incorporation of BrdU into the nuclei during a period of 4 h was used to determine the proliferation of the embryonic cells in vitro. The results show for the first time the DNA synthesis in trophoblast cells of roe deer blastocysts from October to December. During the period of October to ...
The incidence of metaphases with banded chromosomes is shown. Bands were visible in the chromosomes of 52-60% of the cells after BrdU incorporation, whereas very few control cells (4-10%) showed any bands; in the latter case, all of the bands were from early metaphase stages....
Moreover,bothdirectand indirectimmunofluorescence canbeused、TheBrdU incorporation is moreevident usingtheindirectmethod. A、3、2 Anticipated results It is recommanded to perform eachexperiment using a negativeanda positivecontrolsample、 Thenegative sample, inorder tohave a correct setting of instrument,...
benefit from the incorporation of two different analogs at different time points (dual pulse labeling), which can further define cell cycle kinetics. With the availability of new highly specific anti-BrdU antibodies, t...
Application The Cell Proliferation ELISA is designed for use in research studies as a pre- cise, fast, and simple, non-radioactive alternative to quantify cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in proliferating cells by chemiluminescence detection. Thus,...