multiplex, portable, and onsite detection of ASFV. The key technical challenge is to integrate on-chip sample preparation, amplification and detection into an all-in-one system [110]. Although many researchers have made great efforts, the current methods are still limited by the high cost of fa...
Oct 17, 2023 .gitmessage Doc: update basic community files for ASF Nov 24, 2024 .gitmodules Update the googletest module URL May 10, 2024 CODE_OF_CONDUCT.md Doc: update basic community files for ASF Nov 24, 2024 CONTRIBUTING.md Doc: update basic community files for ASF Nov 24, 2024 ...
i) Preparation of the Total Extracts Dry cell pellets of HeLa S3 cells were resuspended in equal volume of the cold lysis buffer [50mM Tri-HCl, pH 7.5, 300mM NaCl, 0,5% NP-40, 10% glycerol, 2mM MgCl2, 10μM CaCl2, 5mM EGTA-KOH pH 8,0, 1mM DTT, phosphatase and protease inhibito...