Instead, it is preferable to have a platform that can take a wide array of mAbs that could be processed to generate a matrix of BsAbs. The variable regions of different Abs can be concatenated to generate a dual variable domain Ab [50]. However optimization of the sequential order and ...
In recent years, antibody engineering has been revolutionised bySNAP-tag technology. A SNAP-tag is a protein that can be fused to any molecule. It can form a link between antibodies that have recognised tumour cells and molecules that diagnose or treat the tumour. The SNAP-tag acts like the...
Quantitative and qualitative analyses of polyclonal plasma were performed to elucidate antibody epitope repertoires using gene fragment-phage display libraries (GFPDL) and surface plasmon resonance (SPR) technology to measure real-time antibody binding kinetics, antibody cross-reactivity, immunoglobulin iso...
Previously, we reported the development of a camelid-human chimeric HcAb, VHH-B11-Fc, developed based on the llama Ab library using phage display technology19,20. This Ab exhibited 100% inhibition of PCSK9 in hepatocellular carcinoma models at a specific concentration (1.5 µM). Moreover, ...
on E. coli, is a tried-and-tested technology for the cost-effective production of proteins including antibody fragments. wacker.com 瓦克获有专利的大肠杆菌分泌技术ESETEC®是一项可靠的生产 蛋 白质 和 抗体 片 段的 低成 本技术。 wacker.com The European Union believed that a more determined...
Skegro D, Stutz C, Ollier R, Svensson E, Wassmann P, Bourquin F et al (2017) Immunoglobulin domain interface exchange as a platform technology for the generation of Fc heterodimers and bispecific antibodies. J Biol Chem 292(23):9745–9759 Article CAS Google Scholar Smith EJ, Olson K, ...
2B). DiD+ Immunoliposome numeration provided using FCM represented 65% (see Supplemental data section for qNano: measurement of liposome concentration) of total Immunoliposomes counted using TRPS technology (qNano). Moreover, considering the PE quantification channel, the positivity threshold was ...
Using our single-cell screening platform technology, CelliGo, we identified a panel of monoclonal antibodies against human CXCR5. We selected HFB2-4, which binds to CXCR5 expressing cells, blocks CXCL13-induced intracellular signaling and B cell migration, and induces ADCC of Raji B cell lymphoma...
Licensing of technology from this laboratory has created over 150 jobs in the biotech/pharma industry. The other authors declare no competing financial interests.Rights and permissions Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (...
, and the Agency for Innovation by Science and Technology (IWT) (to J. W., E. V., and S. E.). This work was also supported by an IWT Ph.D. fellowship (to J. V. d. B.) and FWO-Vlaanderen postdoctoral fellowships (to M. C. and E. S.). This work was also supported ...