与其他人血清蛋白或非胶原蛋白的细胞外基质蛋白的抗胶原抗体的非特异性交叉反应是可以忽略不计。该产品不推荐使用在变性条件下WB,IP,和ELISA。Rabbit Anti-Collagen I antibody 兔抗Ⅰ型胶原抗体我们会建议在自然条件下测试它。变性和还原条件将大大减少这种抗体的反应性和选择性。Abcam不测试样品ab34710 WB内源性。
Immunohistochemistry was performed to test whether fibrinogen and collagen type I were present in the adhesion lesions and exudates. Tissue specimens were fixed for 24 h in IHC Zinc fixative solution (BD Biosciences) and embedded in paraffin wax before sectioning. Sections of 4 µm thickness ...
ab34705 Sheep polyclonal to Malate Dehydrogenase 10mg 3450 ab34706 Rabbit polyclonal to KLH (TRITC) 100ug 3450 ab34708 Sheep polyclonal to Fructosyl Amino Acid Oxidase 10mg 3450 ab34709 Rabbit polyclonal to CCNL1 500ug 3450 ab34710 Rabbit polyclonal to Collagen I 100ug 3450 ab34711 Rabbit ...
Outline Abstract 1. Introduction 2. Materials and methods 3. Results and discussion 4. Conclusion Author contributions CRediT authorship contribution statement Declaration of competing interest Acknowledgements Appendix A. Supplementary data Data availability ReferencesShow full outline Figures (7)...
and ITGAX versus LAIR1 mRNA expression in TCGA LUSC dataset.i–kSplenocytes cultured in vitro on plastic, laminin-rich Matrigel, or collagen were treated with CD18 inhibitory antibody (10 µg/mL) for 96 h and analyzed by FACS for percentage of (i) total CD8+T cells gated from CD...
The kidney has large regenerative capacity, but this is compromised when kidney damage is excessive and renal tubular epithelial cells (TECs) undergo SNAI1-driven growth arrest. Here we investigate the role of IL11 in TECs, kidney injury and renal repair
The following primary antibodies were employed (dilution): collagen I (1:5000, ab34710, Abcam, USA), α-SMA (1:300, ab7817, Abcam, USA), fibronectin (1:1000, ab2413, Abcam, USA), vimentin (1:1000, ab92547, Abcam, USA), E-cadherin (1:500, ab76055, Abcam, USA), p-IκBα (...
The membranes were blocked with 5% non-fat milk and incubated overnight at 4 °C with a primary antibody [anti-collagen type I (ab34710, Abcam), anti-α-SMA; anti-α-Ac-Tub; anti-α-Tub; anti-AT1 (DF7592, Affinity Biosciences); anti-p-MYPT (AF5445, Affinity Biosciences); anti-...