16S rRNA V4 amplicon sequencing of 117 samples (60 ASD and 57 controls) identified 21 amplicon sequence variants (ASVs) that differed significantly between the two cohorts: 11 were found to be enriched in neurotypical children (six ASVs belonging to the Lachnospiraceae family), while 10 were ...
2021. Children with autism and their typically developing siblings differ in amplicon sequence variants and predicted functions of stool-associated microbes. mSystems 6:e00193-20. https://doi.org/10.1128/mSystems.00193-20. The review history of this article can be read here....
of as little as 1 nucleotide. In several mock communities, DADA2 identified more real variants and output fewer spurious sequences than other methods. We applied DADA2 to vaginal samples from a cohort of pregnant women, revealing a diversity of previously undetectedLactobacillus crispatusvariants. ...
生成OTU/ESV:序列去冗余、聚类clust_otu(OTU)或去噪unoise3(ESV, Exact Sequence Variants) OTU筛选:嵌合体生成原理及去除方法、去除线粒体、叶绿体和宿主非特异扩增污染、生成代表性序列和OTU表 物种注释及进化树构建 常用Alpha多样性指数计算 常用Beta多样性距离矩阵计算 ...