Reprogramming of amino acid metabolism, sustained by oncogenic signaling, is crucial for cancer cell survival under nutrient limitation. Here we discovered that missense mutant p53 oncoproteins stimulate de novo serine/glycine synthesis and essential amino acids intake, promoting breast cancer growth. Mecha...
and cleavage rate of DOs; and (3) serine, glutamine, aspartic acid or alanine, and CCs viability. In conclusion, urea significantly changed the turnover of AAs by COCs, CCs and DOs, and reduced the subsequent developmental competence of bovine oocytes. ...
The analysis of dnaC alleles that preserved viability under elevated expression revealed an N-terminal domain of DnaC involved in binding to DnaB. Mutant proteins bearing single amino acid substitutions (R10P, L11Q, L29Q, S41P, W32G, and L44P) that reside in regions of predicted secondary ...
Despite their therapeutic benefits, the precise composition of the transplanted hiPSC secretome in vivo has not been established. While the extracellular microenvironment and interaction with other cell-types (in complex tissues) have been known to modulate the stem cell secretome, most studies have ...
1. A method for determining at least part of the amino acid sequence of a protein; the method comprising: cleaving the protein into proteolytic peptides, ionizing the proteolytic peptides to generate peptide precursor ions, dissociating the peptide precursor ions utilizing tandem mass spectrometry in...
of the two ligands. The larger size of both AuNPs and protein makes the ligands to only interact with the surface active site of proteins3. AS5 binding site, located at the interface between subdomains IIA and IIB of BSA (Fig. 4a), had been proved as the pocket for small molecule, ...
Gd(Ⅲ)-aminopolycarboxglicacid complexes is usually used as contrast agent of MRI.In the this paper, We study the interaction between Gd(Ⅲ)-aminopolycar boxglicacid complexes and BSA by UV. The results indicate there is electrostatic action between them, and this kind of is different with...
acid (2,4,5-T), 4-benzoylbenzoic acid (4-BBA),okadaic acid from Prorocentrum concavum, picrylsulfonic acid(TNBS) solution 1MinH2O, bovine serum albumin (BSA), andalkaline phosphatase labeled antimouse IgG (Fc specific)antibodies developed in goat were obtained from Sigma-Aldrich,France. 2,4...
Amino acid (AA) availability is a robust determinant of cell growth through controlling mechanistic/mammalian target of rapamycin complex 1 (mTORC1) activity. According to the predominant model in the field, AA sufficiency drives the recruitment and activation of mTORC1 on the lysosomal surface by ...
a deprotection of a carbamic acid ester of formula III wherein R1and n are as defined above and RPis an amino protecting group. In a yet another embodiment of the present invention, provided is a pharmaceutical composition, comprising a therapeutically effective amount of a compound of formula ...