In another approach 5′-phosphate prodrugs of A2A agonists have been prepared (e.g. 24) which are to be preferably cleaved releasing the A2A agonist 25 at sites of inflammation where ecto-5′-nucleotidase (CD73) is highly expressed [45]. 2.3 A2B-selective agonists The SAR of adenosin...
The ecto-5′-nucleotidase (E.C. 3.1.3.5) catalyzes the dephosphorilation of AMP producing adenosine and inorganic phosphate18. In fact, the purinergic system is also a direct target of ethanol exposure19,20,21,22,23,24. Both enzymes are affected by acute or chronic exposure to ethanol in...
Cells were grown in the mid-log phase without antibiotics for 18 h before the day of the experiment and washed with phosphate-buffered saline (PBS)/5 mM EDTA, followed by gentle detachment with accutase (Sigma-Aldrich, St. Louis, MO, USA). Cells collected from the culture plates were ...
Ischemia is characterized by a transient, insufficient, or permanent interruption of blood flow to a tissue, which leads to an inadequate glucose and oxyge