Using 100ng/mL phorbol 12-myristate 13-acetate (PMA), THP-1 cells were induced to differentiate into THP-1-derived macrophage-like cells (TDMs). Both MDMs and TDMs were subsequently infected with the Mtb strain
THP-1 and U937 cells were cultured in RPMI supplemented with 10% FCS. HBEC3-KT cells were obtained from ATCC and were grown in Bronchial/Tracheal Epithelial Cell Growth Medium (Cell Applications, Inc.). HaCaT cells were obtained from CLS Cell Lines Service GmbH, and were grown in DMEM ...
Macrophage differentiation and cell co-culture THP-1 was induced into M0 macrophages by adding 100 ng/ml of fopperol 12-myristate 13-acetate (PMA, Santa Cruz Biotechnology, Dallas, USA) to THP-1 cells, followed by incubation for 48 h at 37 °C in a 5% CO2 incubator. The cells were wa...
In advanced breast cancer, macrophages exhibit M2 polarization, secrete arginase-1 and other pro-oncogenic inflammatory factors, and inhibit the antitumor effects of CD8 + T cells [10], [11]. Macrophage-derived IL-6 activates the tumor cell Jak/Stat signaling pathway and activates miR-506-3p ...
Chemokine array identified CXCL1 as a crucial component in EV-dead to activate TAM/PD-L1 signaling. CXCL1 knockdown in EV-dead or macrophage depletion significantly inhibited EV-dead-induced TNBC growth and metastasis. Mechanistic investigations revealed that CXCL1EV-dead enhanced TAM/PD-L1 ...
Further mechanistic studies indicated that NL-SDT enhanced macrophage differentiation toward the M2 phenotype by activating the reactive oxygen species (ROS)–5′ AMP-activated protein kinase (AMPK)–mammalian target of rapamycin complex 1 (mTORC1)–autophagy signaling pathway in murine BM-derived M1 ...
1i). After LPS treatment, the levels of RasGRP2/3/4 were all decreased while RasGRP1 was induced. We also examined the expression profiles of RasGRP1–4 in human THP-1 monocytic cells treated with phorbol-12-myristate-13-acetate (PMA) and human peripheral blood mononuclear cell-derived ...
indicating that CXCL1 expression was closely correlated with EMT process. Meanwhile, ARG1 expression was also increased in the lung metastasis lesions, implying that the enhanced CXCL1 expression might be correlated with increased M2 macrophage phenotype. (All values from three independent experiments are...
Insulin-like growth factor binding protein 2 (IGFBP2) plays crucial roles in cell proliferation, differentiation and more. However, the role of NPs-derived MSCs (PO-MSCs) and IGFBP2 in NPs pathogenesis remains poorly defined. Herein, primary human nasal epithelial cells (pHNECs) and MSCs were...
Isoforms of Jun kinase are differentially expressed and activated in human monocyte/macrophage (THP-1) cells J. Immunol., 166 (2001), pp. 5646-5653 CrossrefView in ScopusGoogle Scholar 25 J.Y. Yang, N. Moulin, M.X. van Bemmelen, G. Dubuis, T. Tawadros, J.A. Haefliger, G. Waeber...