What is the function of a buffer solution in chemistry?SolutionA solution is a homogeneous mixture of components and substances whose reactions at the molecular or ionic level do not react with each other. Chemically a solution is conceived as the mixture between a solvent and several solutes....
<p>To determine which pair of solutions can form a basic buffer solution, we need to understand the components required for a basic buffer. A basic buffer is typically made by mixing a weak base with the salt of its conjugate acid.</p><p>1. <strong>Ident
Calculate: pH value of a buffer solution obtained by: (1) Mixing: 300 ml of NH_4Cl solution where C(NH_4Cl) = 0.1 mol/L with 150 mL NH_3 (2) H_2O solution where C(NH_3) = 0.2 mol/L (3) K(NH_3 * H_2O) ...
Piranha solution, comprising a 2:5 mixture of concentrated hydrogen peroxide (H2O2; 30%, analytic grade, Merck) and sulfuric acid (H2SO4; 95–98%, analytical reagent grade (AR), Bio-Lab), was used for cleaning the electrochemical cells, glassware and Pt electrodes before use. Acetone (AR...
The suspension of protoplasts was loaded at the bottom of a centrifuge tube and covered with 2 volumes of a mixture (1:2, medium A:medium B) (medium B: 0.4 m betaine, 30 mm KCl, 20 mm HEPES-KOH, pH 7.5) and 1 volume of medium B. The gradient was then centrifuged at 1500 × ...
However, most BBB models display a low transendothelial electrical resistance (TEER), which is a measure of the tightness of the BBB. To address these issues we have established and optimised a robust, simple to use in vitro BBB model using porcine brain endothelial cells (PBECs). The PBEC ...
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Localized ginsenoside hydrolysis occurs in the lesion area of P. notoginseng leaves with round spot disease in open field Round spot caused by the necrotrophic fungus M. acerina is a primary foliar disease occurring in cultured P. notoginseng. Given its high prevalence and severe consequences, we...
After 10 min, the reaction mixture was neutralized with phosphate buffer (NaH2PO4, 1.0 M, 2.5 mL), filtered through a layer of Celite, and rinsed thoroughly with ethyl acetate (20 mL). Then the organic layer was dried with anhydrous sodium sulfate and concentrated in vacuo. The obtained ...
After incubation at 25 °C for 15 min, the reaction was terminated by adding 200 μL of 0.2 mol/L ZnSO4 and 200 μL of 0.2 mol/L Ba(OH)2. The mixture was centrifuged and the supernatant containing unreacted 3H-cAMP was transferred to 2.5 mL of scintillation cocktail. Radioactivity was...