在 10 min 内缓慢 注射 1 μ A 25235 ( 5 nmol/μ , Sigma ) , 留针 20 min 。 l β l 手术对照组注射 1 μ 生理盐水 。 l 113 免疫组织化学染色 正常大鼠 ( n = 3 ) , 以及 β 两组杏仁核注射大鼠分别在术后 7 d ( A 25235 组 n = β 6 , 手术对照组 n = 5) 和 21 d ( A...
Organic solvents 2,2,2-trifluoroethanol (TFE), chloroform, and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich Chemie Gmbh (Schnelldorf, Germany). The lipid was dissolved in an organic solvents mixture of chloroform:TFE = 1:1 (by volume) in glass vials at the total ...
Reagents: Aluminum chloride, sodium nitrite, sodium hydroxide, TPTZ, iron (III) chloride solution, ammonium iron (II) sulphate, Folin–Ciocalteu’s phenol reagent, thiazolyl blue tetrazolium bromide, 2′,7′-dichlorofluorescin, and DPPH were purchased from Sigma Aldrich. The amyloid beta peptides ...