To examine LPS-induced cytokine responses over time, wild-type (C56BL/6J) mice were injected intraperitoneally with saline or LPS, and peripheral blood samples were collected at 0, 2, 4 and 6 h post-stimulat
Bronchoalveolar lavage (BAL) was collected with 0.5 ml PBS and red blood cells were lysed with ACK buffer for 2 min at 4 °C, as previously described49. BAL cells were re-suspended in 0.3 ml RPMI-1640 containing 10% FBS, and cell counts were performed using a hemocytometer,...
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Epithelial dendritic cells vs. Langerhans cells: Implications for mucosal vaccines 2024, Cell Reports Citation Excerpt : LCs are derived from the yolk sac during embryogenesis but can be reseeded from blood monocyte precursors. They are found solely in the SSE and have recently been subdivided into...
it is most likely that the failure to obtain a result could be explained by the presence of mutations in the DNA template that prevented the annealing of one or both of the two primers. The complete consensus sequences of A1 and A2 samples were aligned with each other and with the partia...
eFORGE: identify cell type-specific signals in differentially methylated positions (mostly important for blood-based EWAS) http://eforge.cs.ucl.ac.uk/ Model for EWAS using probe signal intensities: http://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-016-1347-4 Reference-based tissue...
cancers Article SP1 and STAT3 Functionally Synergize to Induce the RhoU Small GTPase and a Subclass of Non-canonical WNT Responsive Genes Correlating with Poor Prognosis in Breast Cancer Emanuele Monteleone 1,† , Valeria Orecchia 1,†, Paola Corrieri 1, Davide Schiavone 1,‡, Lidia Avalle...
This work was supported by PGA grants HL66682 and HL66642 from the National Heart, Lung, and Blood Institute (NHLBI), with additional support from NHLBI grant MH59520 (to L.K.) and National Institute of Environmental Health Sciences grant ES-15478 (to D.N.). L.K. is a James S. ...
Compared to HFHS-fed wildtype mice, HNF4α HET mice had down-regulation of lipid catabolic genes, induction of lipogenic genes, and increased hepatic and blood levels of lipids, whereas HNF4α KO mice had fatty liver but mild hypolipidemia, down-regulation of lipid-efflux genes, and induction...
blood CD34+cells from patients with MPN transduced with shRNA targetingTP53or shCTR.n = 5 patients, three independent experiments. Barplot indicates mean ± s.e.m. and two-tailed pairedt-testPvalue (related to Extended Data Fig.5n).k, Schematic representation of the key analytical ...