用途:5×SDS-PAGE蛋白上样缓冲液 纯度:99% 产地:湖北 品牌:卡诺斯 包装规格:10ml/瓶 货号:sj1074 Size:5mL | 10mL | 25mL 有效期:24个月 存储:冰袋运输,-20℃保存,避免反复冻融,建议分装冻存。 产品简介 5×SDS-PAGE 蛋白上样缓冲液(SDS-PAGE Sample Loading Buffer),是一种经过改良的以溴酚蓝为染料...
SDS-PAGE SAMPLE LOADING BUFFER (5*) {SDS-PAGE蛋白上样缓冲液(5*)} CBNumber: CB314211873 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商1 SDS-PAGE SAMPLE LOADING BUFFER (5*) {SDS-PAGE蛋白上样缓冲液(5*)}化学性质 ...
2×蛋白上样缓冲液(非变性)价格:￥50.00/瓶产品详情:2×蛋白上样缓冲液(非变性)英文名称Native SDS-PAGE loading buffer, 2×中文名称2×蛋白上样缓冲液(非变性)别名SDS-PAGE Samp... 纯度:99% 包装规格:5ml 用途:本产品适用于SDS-PAGE(SDS变性聚丙烯酰胺凝胶电泳)时作蛋白质上样用。其主要成份为SDS,溴酚...
联系电话:027-65317797 13667154760 电子邮件:1096814016@qq.com CB指数:58 网址:www.peptidechip.com/ 相关信息:产品目录(10020)用户评价 中文名称:SDS-PAGE Sample Loading Buffer (5*) {SDS-PAGE蛋白上样缓冲液(5*)} 其它信息:纯度:1ml;5ml;50ml | 包装信息:1ml;5ml;50ml...
SDS裂解液 50ml/100ml R21855-500 Tris-甘氨酸电泳缓冲液(5×) 500ml T15267-500 Tris-甘氨酸电泳缓冲液(10×) 500ml T15196-100 Triton X-100细胞裂解液 100ml T15202-05 TruPAGE LDS蛋白上样缓冲液(4×) 5ml T15204-500 TruPAGE转移缓冲液(20×) 500ml T15203-500 TEA-Tricine-SDS...
TCA was added to the eluates to a final concentration of 5% and protein pellets were dissolved in SDS sample buffer and analyzed on Tris-Tricine gels followed by immunoblotting or analysis by mass spectrometry (Fig. 2a). Mass spectrometry Crosslinked samples were diluted in NuPAGE SDS sample ...
All experiments were repeated at least three times. Immunoblotting analysis Immunoblotting was performed with a SDS-PAGE Electrophoresis System as described previously14. Briefly, samples were run on SDS-10% polyacrylamide gels and transferred to nitrocellulose membrane. The membranes were blocked in 5%...
Reaction was stopped by adding SDS sample buffer or by freezing at −20°C. Animal Experiments Animal studies were performed according to protocols approved by the University of Michigan or Duke University. See Supplemental Experimental Procedures for details. See Supplemental Experimental Procedures ...
Proteins were then heated at 95 °C for 10 min in SDS-PAGE sample buffer (62.5 mm Tris-HCl at pH 6.8 containing 2.5% SDS, 5% β-mercaptoethanol, and 10% glycerol) and subjected to SDS-PAGE analysis with 12% polyacrylamide gels. After electrophoresis, proteins on the gels were electro...
本产品作为蛋白质电泳Loading Buffer,适用于变性聚丙烯酰胺凝胶电泳时的蛋白样品制备和上样。蛋白上样缓冲液(SDS-PAGE loading buffer)是一种以溴酚蓝为染料,5 倍浓缩的SDS-PAGE 凝胶电泳上样缓冲液,用于常规的SDS-PAGE蛋白电泳样品上样。 操作步骤: 1. 按每4μL蛋白样品加入1μL 5×SDS-PAGE 蛋白上样缓冲液...