5α-DHT was separated in a second TLC system and definitively identified by recrystallization to constant specific activity.doi:10.1016/0016-6480(83)90108-9H.U. ThiesH.-J. HorstE. HaaseElsevier Inc.General and Comparative EndocrinologyH.U. Thies , H.-J. Horst , E. Haase . (1983) ...
1. Precision pipettes to dispense 50, 100, 150 and 300 μl2. Disposable pipette tips3. Distilled or deionized water4. Plate shaker5. Microwell plate reader with a filter set at 450nm and an upper OD limit of 3.0 or greater* (see Assay Steps step 10).Cat. No.:DEIAPkg.Size...
Testosterone and 5 alpha-DHT stimulated the proliferation of human facial sebocytes in a significant dose-dependent manner. In our system 5 alpha-DHT exhibited the strongest effect; on the contrary, the proliferation of non-facial sebocytes was inhibited by testosterone, whereas 5 alpha-DHT enhanced...
(DHT) (Takahashi et al. Mol. Cell Endocr. 35:15,1984).The purpose of the present work was 1)to determine wether such cartilage cells contain specific binding sites for DHT 2)to study the variation of the 5αreductase activity at different ages from birth to post puberty.The 3H-DHT and...
5α-dihydrotestosterone (DHT, 25 nM) alone did not affect any significant changes in cell viability and in nuclei of FaO cells; however, pre-treatment with DHT protected genomic DNA degradation induced by TGF-β1 for 14 hr. Simultaneous treatment with DHT plus TGF-β1 (D + T) inhibited ...
IL-6- or IL-8-induced cell proliferation was completely blocked by their specific neutralizing antibodies, which partially inhibited DHT-induced cell growth. In the absence of androgen, both cytokines enhanced AR expression and AR promoter activation, which was completely blocked by Flu. However, ...
In the present study, we investigated the effects of KT and DHT on fathead minnow juveniles (Pimephales promelas), with the aim to compare the effects of the two androgens on critical physiological processes, such as somatic growth, male secondary sexual characteristics expression, and gonad ...
Nuclear localization of 尾-catenin promotes myogenesis, inhibits adipogenesis and is necessary for muscle regeneration and hypertrophy Unilateral implantation of DHT pellets in the tibia bone compared to contralateral placebo revealed no local AR-mediated anabolic action. Analysis of hepatic gene expression ...
Interestingly, bcl-2 protein expression was decreased in T- and 5alpha-DHT-treated cells, leading to an increase in the bax/bcl-2 ratio. In addition, T and 5alpha-DHT induced proteolytic cleavage of caspase 8 and inhibited proliferation of DPC at 10(-5) M. High concentrations of T and...
We recently showed that androgen receptors are expressed in Dupuytren's contracture. The aim of the present work was to test the responsiveness of Dupuytren's fibroblasts to 5α-dihydrotestosterone (5α-DHT), the active form of testosterone. Cultured palmar fascia cells from 10 patients with ...