嘌呤霉素(Puromycin)是由白黑链霉菌(Streptomyces alboniger)发酵代谢产生的一种氨基糖苷类抗生素,通过抑制蛋白质合成而杀死革兰氏阳性菌,各种动物和昆虫细胞。某种特殊情况下有效作用大肠杆菌。作用机制在于嘌呤霉素是氨酰-tRNA分子3’末端的类似物,能够与核糖体的A位点结合并掺入到延伸的肽链中。嘌呤霉素同A位点结合后...
Cells were treated with 25 µg/mL phytohemagglutinin (PHA) as a positive control and 0.4% dimethyl sulfoxide (DMSO) as a negative control. Cells and stimuli were removed, and the plate was washed prior to incubation in the detection antibody (7-B6-1-Biotin Cat. No. 3421M-4HPW, ...