In enzymology, a 3alpha-hydroxysteroid dehydrogenase (B-specific) (EC 1.1.1.50) is an enzyme that catalyzes the chemical reaction: Androsterone + NAD(P)+ rightleftharpoons 5alpha-androstane-3,17-dione + NAD(P)H + H+. The 3 substrates of this enzyme are androsterone, NAD+, and NADP+,...
In enzymology, a 3alpha-hydroxysteroid dehydrogenase (B-specific) (EC 1.1.1.50) is an enzyme that catalyzes the chemical reaction: Androsterone + NAD(P)+ rightleftharpoons 5alpha-androstane-3,17-dione + NAD(P)H + H+. The 3 substrates of this enzyme are androsterone, NAD+, and NADP+,...
6) 3-Hydroxysteroid dehydrogenase 3-羟甾类脱氢酶类 补充资料:特异性 分子式: CAS号: 性质:指酶-底物、抗原-抗体、配基-受体之间的相互辨别和选择性结合反应,从立体结构角度上说就是相应的反应物之间构象的对应性。例如,所谓酶的特异性是指一种酶能在两种或多种不同底物之间作出辨别,并与其中构象最合适的一...
PURPOSE:To produce 3alpha-hydroxysteroid dehydrogenase (3alpha-HSDH) advantageous for determining bile acid in organism humor, by cultivating a bacterium belonging to the genus Nocardia, capable of producing 3alpha-hydroxysteroid dehydrogenase. CONSTITUTION:A bacterium (e.g. Nocardia sp. No. Ch2-1 ...
The 3 α-hydroxysteroid dehydrogenase (3 α-hydroxysteroid: NAD(P) oxido-reductase, EC 1.1.1.50) obtained from the soluble fraction of rat liver was purified by centrifugation, ammonium sulfate precipitation, gel filtration and DEAE-cellulose chromatography. The NAD- and NADP-dehydrogenating and tra...
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(1982). Separation and determination of bile acid by high-performance liquid chromatography using immobilized 3a-hydroxysteroid dehydrogenase and electrochemical detector. J. Chromatogra. 239: 773-783. Kamada S., Maeda M., Tsuji A., Umezawa Y., Kurahashi F., Separation and determination of ...
steroiddehydrogenase(3a-HSD)fromPseudomonassp. B-0831,isamemberoftheshort-chaindehydrogenase/ reductase(SDR)superfamily.Transient-phasekineticstud- iesusingthefluorescencestopped-flowmethodwerecon- ductedwith3a-HSDtocharacterizethenucleotidebinding mechanism.Thebindingofoxidizednucleotides,NAD + , NADP + an...
PURPOSE:To produce 3alpha-hydroxysteroid dehydrogenase having high stability, by cultivating a microorganism, belonging to the genus Cellulomonas and having the ability to produce the 3alpha-hydroxysteroid dehydrogenase having specific properties. CONSTITUTION:A microorganism, belonging to the genus Cellulomonas...
Rat liver 3 alpha-hydroxysteroid dehydrogenase cDNA was previously cloned by us. In this study, we used the rat cDNA as the probe to screen a human liver lambda gt11 cDNA library. A total of four different cDNAs were identified and sequenced. The sequence of one of the cDNAs is identical...