竞价结果详细(20190200013)---山东农业大学 申购单主题: 2×TaqPlusMasterMix(DyePlus);2×PhantaMaxMasterMix;FastPurePlasmidMiniKit;FastPureGelDNAExtractionMiniKit 申购单位: 生命科学院 经费科目: 科研专款或基金(纵向) 是否含税: 是 使用币种: 人民币 审批
This product is a mixture of Taq enzyme, dNTPs, MgCl2 and reaction buffer required for PCR reaction. The 2×Taq Master Mix is optimized for conventional PCR amplification reaction, and the amplification length is up to 8KB, which can efficiently amplify the fragment length of 4KB and below. ...
Catalog #: P211 *Package Size: 5× 1 ml 15 × 1 ml 50 × 1 ml Quantity: Buy in bulk and save For larger amounts, pleaseEmail usfor a quote. Product Description Product Manual Product Introduction 2× Taq Plus Master Mix contains Taq Plus DNA Polymerase, dNTPs, and an optimized buffe...
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cDNA was synthesised via reverse transcription, and mRNA levels were examined using 2xTaq PCR Master Mix (Wuhan Huaruikang Biologic Technology Co., Ltd.). The results were normalised with β-actin, and qRT-PCR was performed using real-time fluorescent quantitative PCR (Thermo Fisher Scientific, ...
1 材料与方法 1.1 材料 1.1.1 试剂与设备 DNA 聚合酶 Ex Taq MasterMix 和 PrimeSTAR Max Premix 购自 TaKaRa Bio(Kyoto, Japan).DNA 连接酶试剂盒 Ligation Mix, 限制性 核酸内切酶 Esp3 I(BsmB I)和 Eco31 I(Bsa I)购自 Thermo Fisher Scientific(Waltham, Massachusetts, USA). 本研究培养大肠杆菌...
Reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time PCR (qRT-PCR) were carried out with 2× Taq Master Mix (Dye Plus) on a SimpliAmp System (Thermo Fisher) and with SYBR Green Supermix on an ABI StepOne Plus System. For qRT-PCR, relative gene expression ...
(10 μmo1/L), 12.5 μL 2 × Taq PCRMasterMix (constituent: 0.1 UTaqpolymerase/μL, 500 μM dNTP each, 20 mM Tris-HCl, pH 8.3, 100 mM KCl, 3 mM MgCl2, and stabilizers), and nuclease-free water 8.5 μL. After initial denaturizing at 95°C for 5 min, the reaction mixture ...
The Cas12g1 non-coding plasmid pACYC-Cas12g1 (Addgene plasmid, 120880) was used as a template for PCR amplification of the AbCas12g tracrRNA sequence with Cas12gtracrRNA F and R primers (Supplementary Tables 1 and 3) in 2× Taq Master Mix (New England Biolabs). The non-coding ...
cDNAs were amplified by qPCR reaction using GoTaq® qPCR Master Mix (Promega) and the reaction was carried out in BioRad-iQ-iCycler. The specific primer pairs are listed in Table 1. The results were analysed with CFX Manager software (Biorad) and the relative amounts, obtained with the...