The experiment was performed at least three times using SYBR Green Master Mix (11184ES03, Yeasen, China) to quantify the mean values of delta Ct and SEM (Standard Error of Mean). The primers used for quantification were listed in Supplementary information, Table S1. Protein purification The ...
RT-PCR was conducted using the one-step Super Script III with Platinum Taq DNA Polymerase (Thermo Fisher, USA) and TaqPath COVID-19 kit (Applied Biosystems, USA) on the QuantStudio 3 Real-Time PCR instrument (Applied Biosystems, USA) and 7900 HT ABI machine. The primers and probes used ...
Additionally, as the dosage selection should include 100 times the dose of human exposure in subchronic and chronic toxicology experiments, doses were set in 10 fold increments to a maximum concentration of 500 mg/(kg·d) in the highest dosage. As such, DEHP exposure concentrations were 0, ...
RNA-dependent RNA polymerases (RdRp) are the major source of mutations in coronaviruses. Although RNA polymerases are essential for viral replication, this class of polymerases has high error rates, leading to the accumulation of mutations in the viral genome over time [7]. For coronaviruses, es...
Delpino MV, Marchesini MI, Estein SM, Comerci DJ, Cassataro J, Fossati CA, Baldi PC: A bile salt hydrolase of Brucella abortus contributes to the establishment of a successful infection through the oral route in mice. Infect Immun. 2007, 75 (1): 299-305. 10.1128/IAI.00952-06. Article...
Quantitative real-time PCR and semi-quantitative PCR were conducted using SYBR Green Master Mix (Roche, Germany) and Dream Taq Green PCR Master Mix (Thermo Scientific), respectively. PCR reactions were performed at 95 °C for 40 s, 59 °C for 40 s, repeated within 40 cycles. GAPDH and ...
[22]. In brief, after the reverse transcription reaction, 80 μl of RNase free water were added to each sample. Ten microliters of diluted cDNA product were mixed with 25 μl of SYBR Green JumpStart Taq ReadyMix, 0.5 μl of internal reference dye, and 2.0 μl of specific ...
The Kunming T2DM mice were randomly divided into six groups of 10 mice, and continued to be fed HSHF diets as well as intragastric administration of water (Model groups), GLQ, GLQ.Cr, LUT, MH, or CrY for four weeks at a doses of 100 mg/kg.d., whereas the mice in the Normal ...
RNA copies were quantified from cDNA by a standard curve method qPCR targeting SARS-CoV-2 S gene (Wang et al., 2020), using the TB Green® Premix Ex Taq II kit (Takara). Viral RNA for transfection into HEK293T cells and HPAEpiC were originated from Vero E6 cells infected with SARS-...
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