(Characterizing human fecal microbial communities Methods for V4-16S rRNA gene sequencing and data analysis, calculation of MAZ scores and functional microbiome maturity, and quantification of enteropathogen burden by means of multiplex quantitative polymerase chain reaction (qPCR) are described in the sup...
Pichler M, Coskun OK, Ortega-Arbulu AS, Conci N, Worheide G, Vargas S, et al. A 16S rRNA gene sequencing and analysis pro- tocol for the Illumina MiniSeq platform. Microbiologyopen. 2018;7:e00611.Pichler, M., Coskun, O. K., Ortega-Arbulu, A. S., Conci, N., Worheide, G., ...
引用该文献 Niu M, Zhao Y, Xiang L, Jia Y, Yuan J, Dai X, Chen H. 16S rRNA gene sequencing analysis of gut microbiome in a mini-pig diabetes model.Animal Model Exp Med.2022 Feb;5(1):81-88.doi: 10.1002/ame2.12202. ...
The 16S rRNA gene has been a mainstay of sequence-based bacterial analysis for decades. However, high-throughput sequencing of the full gene has only recently become a realistic prospect. Here, we use in silico and sequence-based experiments to critically re-evaluate the potential of the 16S g...
1. Johnson J S , Spakowicz D J , Hong B Y , et al. Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis[J]. Nature Communications, 2019, 10(1). 2. Pootakham Wirulda,Mhuantong Wuttichai,Yoocha Thippawan et al. High resolution profiling of coral-as...
流程的安装程序与测序数据可从网址(http://www.ligene.cn/win16s/ )免费下载使用。关键词:16SrRNA;微生物组;Linux;QIIME;VSEARCH中图分类号:Q752 文献标志码:A 文章编号:1672-5565(2018)04-239-07Asimplepipelinetoanalyze16SrRNAampliconsequencingdataintheWindowsFANGMeimei,WANGYuxuan,WANGMingyue,ZHENGHelong,...
旋体门。【结论】本试验获得了大额牛瘤胃细菌16SrRNA基因序列,为进一步研究大额牛瘤胃微生态系统组成及 其与饲料消化之间的关系奠定了基础。 关键词:大额牛;瘤胃细菌;16SrRNA基因序列;系统进化分析 PhylogeneticAnalysisofRumenBacterial16SrRNAGene SequencesofGayal ...
High throughput sequencing flow chart 数据库:Silva,GreenGene,RDP 微基生物拥有Silva,GreenGene,RDP三大数据库。其中Silva数据库涵盖了古细菌16S rRNA基因序列及其对应分类信息18797条。 高通量测序 目前市面上常用的用于研究环境微生物的高通量测序平台有Illumina,BGI,PacBio和Thermo Ion。其中Illumina平台的测序读长长...
4.另外,该研究结果显示适当地处理16S rRNA在基因组内的变异有可能提供细菌群落在种水平和菌株水平上的分类学分辨率。 论文ID 原名:Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis 译名:基于16S rRNA基因测序评估在种水平和菌株水平的微生物组分析 ...
^Matsuo, Y., Komiya, S., Yasumizu, Y. et al. Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution. BMC Microbiol 21, 35 (2021). https://doi.org/10.1186/s12866-021-02094-5 ...