2.每支细胞因子配备1ml 复溶buffer,复溶后产品性能更稳定 3.无热源无内毒素EP管分装,从包装上杜绝内毒素干扰 4.冻干粉形式,-20℃可稳定保存至少一年 重组蛋白选择: 公司正在出售的产品: DL-高半胱氨酸DL-Homocysteine 质量规格:0.95 间氟-DL-酪氨酸m-Fluoro-DL-tyrosine 质量规格:0.99 ...
主营产品:生化试剂,标准品,对照品,PCR试剂盒, 核酸检测试剂盒,荧光定.. 公司地址:上海嘉定区澄浏中路3404号 进入商铺收藏商铺 #动态#2025年西格生物春节放假通知 组织来源:肾脏 器官来源:说明书 生长状态:贴壁细胞 品系:细胞系 用途:仅供科研实验 保存条件:55% 基础培养基+40%FBS+5%DMSO ...
尽管pLXSN 16 E6/E7中含有新霉素抗性,但未用G418筛选转导克隆。Southern和FISH分析显示HK-2细胞来源于单克隆。PCR检测证实HK-2细胞基因组中含有E6/E7基因。;细胞传代方法:1:4传代;2-3天换液1次;细胞生长特性:贴壁生长;细胞形态特性:上皮样;相关细胞有:B5537SKIN细胞、BT20细胞、PC-10细胞...
ATP5MD-KD was confirmed by qRT-PCR and western blotting with anti-ATP5MD (1:400 Abcam ab108225). CNNM2-E3-KO was examined by sequencing the target DNA extracted from the transduced cells. Reporting Summary Further information on research design is available in the Nature Research Reporting ...
For detecting the stability in the presence of metal ions (K+, Mn2+, Ag+, Cu2+, Fe2+, Co3+, Zn2+, Ca2+, Mg2+) and other reagents (EDTA and urea), the enzyme was properly diluted with 50 mm Tris-HCl buffer (pH 8.0) containing 5 nm of KCl, MnSO4, AgNO3, CuSO4, FeSO4, ...
Briefly, the last 2 mm of the tails were placed into 75 μL alkaline lysis buffer (NaOH 25 mM, Na2-EDTA 0.2 mM) in a PCR tube. Then, the samples were heated at 95 °C for 20 min. After heating, the samples were cooled at 4 °C, and 75 μL of a neutralization buffer (Tris...
Likewise, molecular tests such as the PCR-based GeneXpert MTB/RIF (Cepheid Inc., Sunnyvale, CA, USA) and the TB loop-mediated isothermal amplification (LAMP) (Eiken; Tokyo, Japan) are costly, and not readily accessible to low-income countries nor suitable for PoC testing [5,6,7]. The ...
(SEQ ID NO:18). PCR amplification of the page library was achieved using a non-specific upstream amplimer (RSP) and P1 each at 0.5 μM final concentration, 1.5 mM MgCl2(Pharmacia Biotech), 1×polymerase buffer (Bohringer Mannheim) and 5 units of Thermus aquaticus DNA polymerase (Boehringer...
主营产品:生化试剂,标准品,对照品,PCR试剂盒, 核酸检测试剂盒,荧光定.. 公司地址:上海闵行区莘福路388弄2号楼 进入商铺收藏商铺 #动态#培养基的制备步骤 #技术#qPCR引物和探针的稳定性长期保持的关键点 用途:仅用于科研 纯度:≥98% 产地:进口、国产 ...
International Journal of Molecular Sciences Article Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells Takato Hara 1, Takayuki Kojima 1, Hiroka Matsuzaki 1, Takehiro Nakamura 1, Eiko Yoshida 1, Yasuyuki Fujiwara 2,...