aCells were washed in PBS 3 times 10 min each and rinsed once in 2× SSC before hybridization. Hybridization was carried out using a Texas Red conjugated 24-mer DNA oligodeoxynucleotide probe (5′-cagatgagccgaatcaaccctggc-3′) complementary to 7SK RNA in a moist chamber at 42°C for 12...
执行命令carcircir-value[pirpir-value] [cbscbs-valuepbspbs-value] {inbound|outbound},在QoS模板中配置流量监管。 缺省情况下,QoS模板中没有配置流量监管。 执行命令remark dscpdscp-value{inbound|outbound},在QoS模板中配置重标记IP报文的DSCP优先级。
1. 贴壁培养的细胞,吸去培养基后,加入10mL/150mm培养板的冷PBS洗两次,每次振摇数次以尽量去除培养液; 2. 悬浮培养的细胞或用细胞刮子刮下的贴壁细胞,将细胞及培养液移至离心管中, 1000转/分离心10 min,再用10mL/150mm培养板的冷PBS,1000转/分离心5 min洗两次; ...
Finally, plates were washed three times with PBS and a high performance chromogenic substrate, 3,3´,5,5´̵tetramethylbenzidine (TMB CORE+, Bio-Rad Laboratories, BUF062) was used to detect HRP activity. The reaction was terminated with 0.5 M H2SO4 and absorbance by the colour ...
成都七中国际部 孙ZY同学获得剑桥大学Girton College心理和行为科学专业（PBS）面试邀请；王RX同学获得剑桥大学Newnham College数学专业面试邀请，2023QS世界大学学科之数学专业排名第2位；巫HL同学获得剑桥大学士地经济专业面试邀请；杨HB同学获得剑桥大学工程专业面试邀请，QS世界大学学科之工程与技术专业排名第3位；夏GH...
PBS: Phosphated buffered saline PCR: Polymerase chain reaction PD: Protein D PHiD-CV: Pneumococcal-Haemophilus influenzae protein D conjugate vaccine POET study: Pneumococcal Otitis Efficacy Trial QoL: Quality of life RCT: Randomised controlled trial RR: Relative risk SAE: Serious adverse...
Subsequently, cells were washed three times with PBS, aliquoted, and chased for 0, 3, and 7 h in DMEM-Medium supplemented with 10% fetal calf serum in the absence or presence of VCP inhibitors ( 5 μM CB-5083 or 5 μM NMS-873). The labeled cells were harvested and lysed ...
性状Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4 复溶Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex ...
依隆大学在帮助学生就业方面也很给力,毕业生中有约 90% 在毕业后一年内找到了与其专业相关的职位,且传媒专业的校友在各大媒体公司找到了工作,包括 NBC、PBS、Oprah Winfrey Network、Jet.com 和 Houston Chronicle。 Syracuse University 图片来...
Environmental swabs were displaced into 9-mL PBS dilution tubes and then split into two. Samples of 10 ± 0.5 g of corn silage were diluted in 90 mL of PBS and then placed in a stomacher for 2 min at 260 rpm (te Giffel et al., 2002) and then split in half into screw-cap tubes...