产品名称 rCutSmart™ 反应缓冲液 产品规格 5 ml 型号 B6004S 用途范围 用于科研 是否进口 是 加工定制 否 英文名称 rCutSmart™ Buffer Concentration 10 X 储存 -20℃ 产地 美国 售卖区域 全国 质检 通过质检 发货地 北京 供货商 北京百奥创新科技有限公司 品牌 NEB 价格说明 价格:商品在...
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英文名称:10×YTM Buffer(For Restriction Enzyme) 保存条件:-20℃ 百奥莱博的10× YTM Buffer是一种能让超过200种限制性内切酶的活性达到100%的缓冲液。使用YTM Buffer,可以免去内切酶酶切时选择buffer的麻烦,特别是在双酶切时会变得更加简单易行。此外,许多DNA修饰酶在YTM Buffer中也保留了100%酶活性,这样在酶...
Description:Product SourceAn E. coli strain that carries the NciI gene from Neisseria cinerea (NRCC 31006).Reagents SuppliedThe following reagents are supplied with this product:Store at (°C)ConcentrationCutSmart® Buffer-2010XNotes:NciI produces D
The reaction includes different enzymes that work together in the same buffer (see Figure 1): The exonuclease creates single-stranded 3´ overhangs that facilitate the annealing of fragments that share complementarity at one end (the overlap region) The polymerase fills in gaps within each ...
NEBuffer 2.1: 50%NEBuffer 3.1: 10%CutSmart Buffer: 100% 特性 CutSmart、重组酶、基因工程改造酶、省时酶、高保真酶。 反应条件 CutSmart 缓冲液,37℃。 浓度 20,000 units/ml。 甲基化敏感性 对哺乳动物基因组 DNA CpG 甲基化敏感。 货号 规格 价格 北京库存 上海库存 广州库存 成都库存 #R3510L 5,...
Figure 4: RNA recovery is increased by incubating the column with the elution buffer (nuclease-free water) prior to the elution spin rRNA (16S and 23S Ribosomal Standard from E.coli, Sigma) was purified using the Monarch RNA Cleanup Kit (500 µg, NEB #T2050). 100 µl of nuclease-fr...
Direct loading, additional loading buffer and heat incubation not required 相关产品 相关产品 Blue Prestained Protein Standard, Broad Range (11-250 kDa) Color Prestained Protein Standard, Broad Range (10-250 kDa) 注意事项 To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading...
The reaction includes different enzymes that work together in the same buffer (see Figure 1): The exonuclease creates single-stranded 3´ overhangs that facilitate the annealing of fragments that share complementarity at one end (the overlap region) The polymerase fills in gaps within each ...
due to its ease-of-use, flexibility and suitability for large DNA constructs.Gibson Assembly efficiently joins multiple overlapping DNA fragments in a single-tube isothermal reaction (1,2). The Gibson Assembly Master Mix includes three different enzymatic activities that perform in a single buffer: ...