1.中文名酚:10%SDS 2.英文名:10%SDS 3.分 类:生化试剂 4.保存条件:4℃ 5.品 牌:solarbio 10%SDS的详细描述 10%SDS仅用作科研试验。 参考文献: 《Long noncoding RNA ENST00000413528 sponges microRNA‐593‐5p to modulate human glioma growth via polo‐like kinase 1》 作者:Ren Zhang, Ruo‐Lun...
The same amount up to 30ug of protein was subjected to 10% SDS-PAGE and transferred to PVDF membranes. After being blocked in 10% skimmed milk, the membranes were incubated with Rab10 polyclonal antibody (1:1000 dilution; cat. no. ab104859, Abcam) at 4 °C overnight, washed 3 times ...
为了进一步研究蒙古黄芪(Astragalus mongholicus Bunge)中的蛋白质及其功能,通过金属离子螯合层析,离子交换层析和凝胶过滤得到一种新蛋白(AmPR-10),并对其部分性质进行了研究.AmPR-10在SDS-PAGE上的分子质量为17.2 ku,凝胶过滤层析测得分子质量为32.6 ku,说明其为同源二聚体.糖蛋白染色显示为糖蛋白,总糖含量为13.7%,...
规格参数: 货号:kx10-1 体积:50ul/500ul/1ml 浓度:1mg/ml. 质量控制: 纯度:经高效液相色谱(SEC-HPLC)和SDS-PAGE检测,纯度大于98.0%. 内毒素:小于1EU/mg. 使用说明: 建议长期-80℃分装保存,无菌条件下操作,避免污染。 具体用量需通过预实验确定。
Finally, the enriched proteins were separated by SDS‒PAGE for analysis by mass spectrometry or Western blotting. RNA immunoprecipitation sequencing (RIP-seq) and data analysis RIP-seq was performed on BxPC-3 cells by SeqHealth Technology Co., Ltd. (Wuhan, China). Briefly, 1 × 107 ...
(10-180kD)产品编号产品名称包装P0068彩色预染蛋白质分子量标准(10-180kD)200µl产品简介: 碧云天生产的彩色预染蛋白质分子量标准(PrestainedColorProteinMarker)包含了从10kD到180kD共10种纯化的预染蛋白质,其中72kD条带为红色,10kD条带为绿色,其余条带为蓝色(见右图),适合作为SDS-PAGE或Western的蛋白质分子量...
RAW264.7 histones were resolved by SDS-PAGE and blotted for detection of histone acetylation (blot was cropped; full length blot is shown in Figure S4) (D). Histones H3 and H4 were excised from the gel and subjected to LC-MS/MS analysis (E). MS-275 or SAHA increased acety...
P1300 SDS-PAGE 凝胶制备试剂盒 PR1700 预染次高分子量蛋白 MARKE 参考文献:《Dexmedetomidine attenuates the neurotoxicity of propofol toward primary hippocampal neurons in vitro via Erk1/2/CREB/BDNF signaling pathways》 作者:Youbing Tu, Yubing Liang, Yong Xiao, Jing Lv, Ruicong Guan, Fei Xiao, Yu...
Cell lysates were mixed with 4 × SDS–PAGE loading buffer [250 mm Tris–HCl, pH 6.8, 8% (w/v) SDS, 40% (v/v) glycerol, 0.02% (w/v) Bromphenol Blue and 4% (v/v) 2-mercaptoethanol] to final total protein concentration of 1 μg/μl and heated at 85 °C for 10 min. Sam...
Phos-tag SDS-PAGE对于蛋白样品中的杂质非常敏感,尤其是螯合剂,钒酸,无机盐,表面活性剂这类物质。 强烈建议在Phos-tag SDS-PAGE之前通过TCA沉淀或渗析法降低杂质含量。 转膜前处理: 另一个必须的步骤是在转膜前,用EDTA去除凝胶中的金属离子(Mn2+或者Zn2+); 该步骤可提高蛋白的转膜效率。 ● 分别准备10 mmol/...