The slides were washed with 1 × PBST buffer solution for 5 min/3 times, and then, the secondary antibody HRP Goat Anti-Rabbit IgG (1:200, abcam, Cat #ab111909) was added and placed at 37 °C for 1 h. After washing the slides at the end of the secondary antibody reaction ...
The incubated proteins were then washed using PBST five times, eluted by boiling in 1 × loading buffer and then analyzed by IB assay. Chromatin immunoprecipitation (ChIP) assay The ChIP assay was performed using the MAGnify Chromatin Immunoprecipitation System (Thermo Scientific) according to ...
After 3 washing steps with PBST, cells were ready to be imaged. Cells can be stored at 4 °C before imaging on the PerkinElmer Opera Phenix automated microscope or on a Zeiss LSM 980 microscope. Imaging quantificationsImages were analyzed using the high-content image acquisition and analysis...
1、ELISA 试剂盒使用说明书（5 孔板格式）1 ELISA kit Instruction Manual 5-plate format July, 2006 For research use only.Not for use in diagnostic or therapeutic procedures.
The plates were blocked in PBS/0.1% Tween 20 (PBST) containing 5% dried skimmed milk. The plates were washed three times in PBST before adding Fc mutants at the indicated concentrations and left at 4 °C overnight. The plates were washed as above and incubated for 2 h with 1:500 ...
The membranes were washed three times with PBST, and immunoreactive proteins were visualized by the enhanced chemiluminescence system (Amersham Biosciences). Immunoprecipitation Assays—Immunoprecipitation and Western blotting were performed as described previously (48). HEK 293T cells were plated at a ...
(PBST; 0.05% Tween 20R ) three times. Detection antibody was added to each well after shaking the whole plate on an orbital shaker at the speed of 700 rpm/min overnight at 4◦C. Each well was washed three times with 0.05% PBST before adding 2X reading buffer. Electrochemiluminescence ...
1.2.7 免疫组化染色 分离4周龄小鼠卵巢组织,进行石蜡切片,常规脱蜡至水,经EDTA抗原修复后;采用3%过氧化氢处理切片以淬灭内源性过氧化物酶活性,PBST漂洗后滴加5% 山羊血清 37 ℃封闭30 min;甩去多余的液体,滴加1∶50稀释的兔抗DNAJB11(Abcam, ab75107)。 4 ℃孵育过夜,PBST漂洗后,滴加1∶400生物素化山羊...
用PBST缓冲液洗涤3次,每次5 min。ECL显色液显影,压X光胶片,常规显影定影。 1.2.6 检测SREBP-1c过表达对HepG2细胞内脂滴的 影响 在稳定表达SREBP-1c及阴性对照的HepG2细胞中加入200 μmol/L的油酸刺激24 h后检测脂滴:①Hoechst 33258细胞核染色。先将1 mg的Hoechst 33258溶解于5 mL的0.01 mol/L PBS中,...
After ABC, slides were washed three times with 1xPBST and final steps of IHC stainings were performed. DAB staining was performed using DAB solution (Company: Vector laboratories, Cat No. SK-4105. One drop of Chromogen in 1 ml of Diluent solution) and allowed to stain for no more ...