TRITON X-100 Immunoprecpitation and Cell Lysis Buffer (TIB-1; 1X, pH 7.4) Highlights Applicable to immunoprecipitation-pull down procedures Maintains charge-charge and hydrogen bonding interactions between proteins, but abrogates hydrophobic interactions ...
Briefly, lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 1 mM DTT, 8% glycerol, Protease inhibitors) lysed the cells in ice for 30 min. After centrifugation, the supernatant was incubated overnight with pre-washed M2 beads rotating at 4...
Rhotekin-RBD Agarose储存溶液:50mM HEPES (pH7.4), 150mM NaCl, 5mM MgCl2, 0.5% Triton X-100, 1mM DTT, 10% glycerol. Lysis Buffer:50mM Tris (pH7.4), 1% Triton X-100, 0.5% Sodium Deoxycholate, 0.1% SDS, 500mM NaCl, 10mM MgCl2, 10μg/ml Leupeptin, 10μg/ml Aprotinin, 1mM PMSF....
Whole-cell lysates were obtained by re-suspending cell pellets in the lysis buffer (50 mMTris pH 7.4, 150 mM NaCl, 1% Triton X-100) with freshly added protease inhibitor (Roche) as previously described [63, 64]. Nuclear proteins were prepared with the NE-PER Kit (Pierce) following the...
产品名称 1×CHAPS Lysis Buffer 产品用途 实验用 用途 实验用 品牌 尚宝 型号 C0721-500 T16405-100 常规溶液 α-萘酚试液(26mg/ml) 100ml C0721-100 常规溶液 1×CHAPS Lysis Buffer 100ml C0721-500 常规溶液 1×CHAPS Lysis Buffer 500ml T16439-100 常规溶液 4×Tris-Hcl缓冲液(1.5M,pH=...
Brain cortical tissue, liver, mBCEC, or pBCEC were lysed by brief sonication in protein lysis buffer (containing 50 mM Tris, 10 mM EDTA, 1% Triton X 100, phosphatase inhibitor (PhosSTOP™), protease inhibitor cocktail (Roche Diagnostics cOmplete™), and pH adjusted to 7.5). Protein...
Cells were lysed in ice-cold whole cell lysis buffer(50 mM Tris-HCl, pH 8.0, 4 M urea and 1% Triton X-100) supplemented with complete protease inhibitor Cocktail (Roche Diagnostics, 04693132001). Whole cell lysates with equal protein were resolved by SDS-PAGE and transferred to nitrocellulose...
T16233-100 甘油溶液(10%) 100ml R22078-100 革兰阴性菌裂解缓冲液 100ml R22079-100 革兰阳性菌裂解缓冲液 100ml R22033-100 Gey's红细胞裂解液(Gey's Lysis Buffer ) 100ml、500ml R21817-500 铬酸钾溶液(100g/L) 500ml T16245-100 HEPES缓冲液(50mM,pH=7.8) 100ml、50...
Cells (3 × 106 cells) were lysed in lysis buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.5 mM 4-(2-aminoethyl)-benzene sulfonyl-fluoride hydrochloride (AEBSF), 2 μg/ml leupeptin, 2 μg/ml aprotinin, 1 μM E-64, 0.5 mM EDTA, 1 mM sodium orthovanadate). The...
Cells were then washed once in ice-cold PBS, harvested using a cell scraper, collected by centrifugation (400 g, 5 min, 4 °C) and lysed in lysis buffer [150 mM NaCl, 50 mM citric acid, 1% Triton X-100, protease inhibitors, 10 mM N-ethylmaleimide (NEM), 0.5% 3-[(3...