aProtein was extracted from cells by incubation with extraction buffer (1 mM CaCl2; 150 mM NaCl; 10 mM Tris, pH 7.4; 1% Triton X-100; 1 mM PMSF plus one tablet of protease inhibitor cocktail (Roche, NJ, USA) per 20 mL buffer) for 5 min on ice. Crude lysate was centrifuged at 13...
Conventional Coomassie Brilliant Blue Stain 常规考马斯亮蓝染色液 Skimmed Milk Powder 封闭用脱脂奶粉 Congo red staining solution (1%) 刚果红染色液(1%) Protease inhibitor cocktail,plant 蛋白酶抑制剂混合物,植物 Magnetic stirrer 磁力搅拌子 (2RS)-Lotaustralin 百脉根苷 1973415-50-7 11...