Polyethylene terephthalate (PET) is the most commonly used thermoplastic polyester on a global scale. Due to its high resistance to various physical and chemical factors, PET waste can persist for a very long period in natural ecosystems where it can cause ser...
从2005年第一个PET水解酶被报道至今十几年的时间里,这个领域的研究包括机制分析、生化表征和蛋白质工程等,一直局限于几种基准酶。由于缺少有效的专门针对PET水解活性的高通量筛选方法,人们无法更全面地检索各种微生物资源,也无法从大规模的突变...
首先,用2株工程化的枯草芽孢杆菌构建了一个双微生物菌群,它们分泌的PET水解酶(PET hydrolase, PETase)和单羟乙基对苯二甲酸水解酶(monohydroxyethylterephthalate hydrolase, MHETase)在7 d内降解13.6%的PET薄膜,另外通过添加红球菌可对中间产物TPA进一步降解,30 ...