开发针对线粒体DNA的基因编辑工具一直是线粒体遗传学领域的长期目标,也是研究和治疗线粒体疾病的有力工具。迈阿密大学米勒医学院和Precision公司的研究人员在 Nature 子刊 Nature Metabolism 上发表了题为:Efficient elimination of MELAS-associated m.3243G mutant mitochondrial DNA by an engineered mitoARCUS nuclease ...
Due to the lack of the complete repair system of DNA damage, a convenient method for detecting activity of mitochondria-targeted nuclease needs to be innovated urgently. Using transgenic technology, a mitochondrial DNA containing two target sequences(T1 and T2)was integrated into host genome ...