本发明以神经元特异性DEC1基因敲除小鼠和DEC1基因过表达小鼠为实验对象,通过小鼠大脑中动脉缺血再灌注损伤造成脑卒中模型进行研究,结果表明与对照组小鼠相比,DEC1基因敲除小鼠梗死体积明显增加,神经功能明显恶化,死亡神经细胞数量增多;DEC1基因过表达小鼠梗死体积明显降低,神经功能明显改善,死亡神经细胞数量减少。体外细胞...
2、分化型胚胎软骨发育基因 1过表达与乳腺癌进程密切相关 ChakrabartiJ等应用免疫组织化学技术对253例正常组织、原位癌及侵袭性乳腺癌 组织芯片进行表达与病理相关性分析研究发现:分化型胚胎软骨发育基因1(DEC1)的过 表达与肿瘤细胞的侵袭性和恶性潜能关系密切,在乳腺癌中表现为:随着肿瘤的进 展,DEC1的表达水平从...
摘要: 目的 构建人分化型胚胎软骨发育基因1 (DEC1)的短发卡RNA (shRNA)慢病毒表达载体并建立稳定敲减DEC1表达的人脑胶质瘤细胞株T98G.方法 根据GenBank中DEC1基因cDNA序列设计合成两条特异性shRNA序列,同时设计1条非特异性序列作为阴性对照,分别克隆到Psi-LVRU6MP质粒载体内,经酶切... 查看全部>> ...
[20] Foust KD, Salazar DL, Likhite S, Ferraiuolo L, Ditsworth D, Ilieva H, Meyer K, Schmelzer L, Braun L, Cleveland DW, Kaspar BK. Therapeutic AAV9-mediated suppression of mutant SOD1 slows disease progression and extends survival in models ...
[4] Drilon A, Siena S, Dziadziuszko R, et al.; trial investigators. Entrectinib in ROS1 fusion-positive non-small-cell lung cancer: integrated analysis of three phase 1-2 trials. Lancet Oncol. 2020 Feb;21(2):261-270. doi:...
RNA干扰(RNA interference,RNAi)是真核生物体内在转录后水平调控基因表达的机制。Dicer是RNAi通路里一种重要的蛋白,它在RNAi的起始阶段通过剪切双链RNA(dsRNA)产生非... 王古樾 - 上海海洋大学 被引量: 0发表: 0年 Construction and screening efficient VEGF siRNA expression vector in zebrafish斑马鱼VEGF基因的si...
METHODS: Totally 23 patients with ischemic stroke were collected from our hospital during Nov.-Dec., 2016. Genotype of clopidogrel related gene (CYP2C19*17, CYP2C19*3, CYP2C19*2, ABCB1 and PON1) were determined by Sanger sequencing method. Clinical pharmacists provide suggestions according to ...
目的分析人浆细胞瘤转化迁移基因(PVT)1的11个单核苷酸多态性(SNPs)与糖尿病(DM)及糖尿病肾病(DN)关系.方法利用Mass ARRAY质谱分析对DM及DN患者进行PVT1基因11个SNP位点(rs11993333,rs1499368,rs1499373,rs2250888,rs2648876,rs2720659,rs2720660,rs2720662,rs2720666,rs2720667,rs3815871)检测,分析各位点多态性在...
found associated with NMDA receptors at synapses. The encoded protein is phosphorylated by calmodulin-dependent protein kinase II and dephosphorylated by NMDA receptor activation. Defects in this gene are a cause of mental retardation autosomal dominant type 5 (MRD5). [provided by RefSeq, Dec 2009...