5.Gamma-H2AX抗体(1:200)(10μl)孵育细胞,4°C过夜。在缠了封口膜的载玻片上孵育,载玻片-封口膜-抗体-细胞-爬片(有细胞那面的朝下),孵一抗二抗都要将载玻片置于孵育盒内,盒底铺纱布,PBS湿润。 6.PBS振荡洗涤3次,每次5min,FITC标记羊抗鼠二抗(1:1000)。室温孵育细胞2h(在暗室操作)。 7.PBS振荡洗涤...
Double-strand breaks are among the first procedures taking place in cancer formation and progression as a result of endogenic and exogenic factors. The histone variant H2AX undergoes phosphorylation at serine 139 due to double-strand breaks, and the gamma-H2AX is formatted as a result of genomic...
gamma-H2AX Antibody (A700-053) in ICC/IF Detection of human gamma-H2AX by immunocytochemistry. Sample: FFPE section of human HeLa cells treated with etoposide. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] (Product # A700-053) (lot 2) used at 1:250. Se...
Trevigen的HT Gamma H2AX Elisa 试剂盒提供包埋有γ-H2AX抗体96孔板来捕获样本中的γ-H2AX,H2AX抗体作为检测抗体,后用山羊抗小鼠HRP标价的二抗与之结合,利用化学发光底物产生的光强度单位即可检测样本中γ-H2AX的量。该试剂盒可用于悬浮细胞、贴壁细胞人外周血单核粒细胞
青云英语翻译 请在下面的文本框内输入文字,然后点击开始翻译按钮进行翻译,如果您看不到结果,请重新翻译!Aging- and Oxidative Stress-related Accumulation of Gamma-H2AX in Drosophila Intestinal Stem Cell选择语言:从 到 翻译结果1翻译结果2 翻译结果3翻译结果4翻译结果5 翻译结果1复制译文编辑译文朗读译文返回顶部...
产品名称:Histone Gamma-H2AX/pH3 Assays 产品型号:SSA-gH2AX 产品展商:XENOMETRIX 产品文档:无相关文档 简单介绍 Histone Gamma-H2AX/pH3 Assays Art. No.: SSA-gH2AX Unit Service Analytics for 1 - X Compounds Histone Gamma-H2AX/pH3 Assays的详细介绍 ...
文2:DNA双链断裂修复蛋白在宫颈癌和CIN组织中的表达放射线主要是通过导致DNA双链断裂(DNA double strand break,DSB)起到杀死肿瘤细胞的作用,但细胞都有不同程度的DSB修复能力,修复方式有两种:以DNA依赖蛋白激酶(DNA dependent protein kinase,DNA PK,包括Ku80/Ku70异二聚体和DNA PK催化亚单位DNA PKcs)为主的非...
Cancer develops due to the accumulation of genetic and epigenetic changes caused by various factors. An important source of genetic changes is DNA double-strand breaks (DBSs). Radiation, oxidative stress, and carcinogens in the environment cause DNA DSBs
Definition A histone variant called H2AX is phosphorylated at serine 139 when double- stranded breaks (DSBs) occur in cells. This phosphorylated form of H2AX is called GammaH2AX, a hallmark for DSBs. BRIT1 GeneThis is a preview of subscription content, log in via an institution to check ...