英文名称 2-Mercaptoethanol 储存温度 2-8°C储存,充氩 运输条件 冰袋运输 产品介绍 2-巯基乙醇是一种硫醇化合物,常用作有机反应中的还原剂。 应用: 2-巯基乙醇广泛用于延迟溶液中的生物化合物氧化。BME 适用于在聚丙烯酰胺凝胶电泳前还原蛋白质二硫键,通常用于 SDS-PAGE 的样品缓冲液,浓度为 5%。 切割分...
Mycoplasma-free MIN6 cells (AddexBio, USA; C0018008) were cultured in Advanced Medium (AddexBio C0003–04) containing 15% (vol./vol.) heat-inactivated FCS (Gibco, USA; 10270106) and 0.05 mmol/l 2-mercaptoethanol (Gibco 21985023) in a humidified incubator at 37°C and 5% CO2. Islet is...
Protein complexes were eluted in 2× sample buffer (100 mM Tris, pH 6.8; 4% SDS; 20% glycerol; 10% 2-mercaptoethanol; 0.2% bromophenol blue) and processed for immunoblotting. ERβ2 and IgG antibodies are detailed in Table S2. The specificity of the ERβ2 antibody was ascertained using ...
Effects of [beta]-mercaptoethanol on the growth of preantral follicles and the maturation of intrafollicular oocytes.(Report)Gong, Seung PyoLim, Jeong Mook
This method provides a useful enzymatic assay for pyridoxal phosphate-dependent beta-replacement reactions in which the amino acid substrate is L-cysteine and the cosubstrate is 2-mercaptoethanol. The assay should be applicable to similar reactions with L-cysteine and other cosubstrates. The method ...
et al. Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines. Endocrinology 130, 167–178, doi: 10.1210/endo.130.1.1370150 (1992). 47. Mosser, R. E. & Gannon, M. An assay for small scale screening of candidate beta cell proliferative factors using intact ...
Animals from Examples 6 and 7, are sacrificed on day 44 and used to prepare single cell suspensions of spleens from control and immunized mice in complete medium, which consists of RPMI 1640 supplemented with 10% FBS, 2 mM L-glutamine, 10 mM HEPES buffer, 50 mM 2-mercaptoethanol and anti...
The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. Lysis Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM...
Defects in insulin processing and granule maturation are linked to pancreatic beta-cell failure during type 2 diabetes (T2D). Phosphatidylinositol transfer protein alpha (PITPNA) stimulates activity of phosphatidylinositol (PtdIns) 4-OH kinase to produce
GC1-spg cells, were cultured in DMEM supplemented with 10% FBS, 2 mM glutamine (Invitrogen, USA), 1% non-essential amino acids (Invitrogen, USA), and 0.1 mM β-mercaptoethanol (Sigma, USA) as previously reported [12]. 2.2. Quantitative real-time PCR (qRT-PCR) Used 10 μL 2× SYBR ...