Analysis of the kinetic properties indicated that RuBGX1 had a lower affinity for pNP-Glc substrate as the Km was 0·164 mmol l1 for pNP-Glc and 0·03 mmol l1 for pNP-Xyl at pH 6·0 and 50°C, respectively. The capabilities of RuBGX1 β-xylosidase for hydrolysis of xylooligosaccharide...
4-Xyl-β-, which is attached to aserineresidue in the protein.131In nature, thebiosynthesisofGAGsis initiated with site-specific transglycosylation from UDP-xylose to proteins catalyzed by twoxylosyltransferaseisoenzymesI and II with recognition of a G-S-G or G-S-X-G site on theamino acid...
Angiosperms possess a retaining trans-α-xylosidase activity that catalyses the inter-molecular transfer of xylose residues between xyloglucan structures. To identify the linkage of the newly transferred α-xylose residue, we used [Xyl-3H]XXXG (xyloglucan heptasaccharide) as donor substrate and reductiv...
cerevisiae, and the glycoside hydrolase family 3 beta-xylosidase gene xyl3A from Penicillium oxalicum was coexpressed in BSPX042. The recombinant strain BSGIBX with the highest extracellular activity of beta-xylosidase was determined by comparing different signal peptides. The hydrolysis of xylobiose ...
Methods and Results: A beta -glucosidase/xylosidase gene for pNP-Xyl at pH 6.0 and 50 degrees C, respectively. The capabilities of RuBGX1 beta -xylosidase for hydrolysis of xylooligosaccharide substrates were further investigated using an endoxylanase-coupled assay. Hydrolysis time courses illustrated...
( 1999 ) Sequence analysis, overexpression, and antisense inhibition of a beta-xylosidase gene, xyl A, from Aspergillus oryzae KBN616 . Appl Environ Microbiol 65 , 20 – 24 .Kitamoto, N., S. Yoshino, K. Ohmiya, and N. Tsukagoshi. 1999. Sequence analysis, overexpression, and antisense ...
A minor β-xylosidase (BXYL II) from Penicillium janczewskii was purified by ammonium sulfate precipitation (30% saturation) followed by DEAE-Sephadex chromatography in pH 6.5 and elution with KCl. The enzyme presented molecular weight (MW) of 301 kDa estimated by size exclusion chromatography...
structure, it is likely that the enzymatic differences between Ppxyl43A and Ppxyl43B might be related to the extra C-terminus domain (GH43_C2) in Ppxyl43B, which could enhance the enzymatic stability while restricting the substrates' or metal ions' access to the active sites of Ppxyl43B. ...
An intracellular beta-xylosidase (AbXyl), from the thermoalkaline Anoxybacillus sp. 3M, was purified and characterized. The homodimeric enzyme (140 kDa) was optimally active at 65 degrees C and pH 5.5, exhibited half life of 10 hat 60 degrees C, 78 and 88% residual activity after 24 h,...
An endogenous beta-xylosidase gene, xyl3A, was overexpressed using two types of promoters in cellulolytic P. oxalicum RE-10. The mutants RXyl, RGXyl-1 and RGXyl-2 displayed higher beta-xylosidase production than native strain RE-10 besides higher cellulase and xylanase activities, especially R...