MA5-15452 targets beta-Actin in indirect ELISA, FACS, IF, and WB applications and shows reactivity with Hamster, Human, mouse, Non-human primate, and Rat samples. The MA5-15452 immunogen is synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH...
肌动蛋白 内参抗体 產品图片 GTX124214 WB Image Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX124214) diluted at 1:15000. ...
GTX109639 WB Image The data was published in the 2020 in Neural Plast.PMID: 33082778 GTX109639 WB Image The data was published in the 2020 in Viruses.PMID: 33003584 GTX109639 WB Image The data was published in the journal Int J Mol Sci in 2019.PMID: 31744099 ...
beta-Actin抗体做wb时,一抗和二抗浓度是怎么确定的?怎么设置抗体滴度: 一抗的用量和样品中的目标分子含量相关,如果含量较高,就不需要用太多一抗,以免浪费和条带太亮不易分析。推荐采用厂家的抗体浓度(义翘神州会提供抗体用量、天然细胞株检测结果),如果样品与厂家的不是一种细胞,可以尝试上下摸索一两个浓度梯度;...
WB IP IF 1:10000-1:40000 1-4 μg 1:200-1:1000 小鼠β-Actin单克隆抗体使用案例 抗体对比实验 ✦样本:人HEK293T细胞、小鼠C2C12细胞、大鼠C6细胞、猪PK15细胞 ✦上样量:每孔10 μg蛋白 ✦一抗:国外知名P公司beta Actin抗体或本beta Actin抗体,分别稀释到终浓度0.1 μg/mL ✦二抗:Goat Anti-...
来源:Rabbit 克隆类型:Polyclonal浓度:1mg/ml亚型:IgG应用: WB=1:1000 ELISA=1:1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 IP=1:100 Flow-Cyt=1:100-200反应: Human 免疫原:KLH conjugated synthetic peptide derived from beta-Actin保存:-20℃保质期:1年 相关标记抗体:HRP标记抗体,Biotin...
抗Beta-Actin(ACTB)鼠单克隆抗体,稀释比过一万,拓然生物销售过百支的经典Beta Actin内参抗体,上海拓然生物是Proteintech现货小包装抗体在上海地区的授权一级代理。Applications Tested applications ELISA, WB,
而且有些文章在做wb的时候,好像没有内参图?我不知道他们是怎末确定上样量的准确的,单纯依靠测出来的上样蛋白浓度?? eric930(2014-2-03 21:51:46) GAPDH也不能用于你的实验,因为它一般主要用在the whole cell lysate和cytoplasmic proteins. 我个人意见是因为你只想要检测NF-Kappa B在核内的表达,就直接用nuc...
初次进实验室,做了几次western blotting,用beta-actin做内参。上样之前使用UV750测定总蛋白浓度。奇怪的是每次beta-actin 总是两条甚至三条平行的条带,上样量也不均一。我的具体操作过程如下: 1、目标蛋白分子量70kD,10%分离胶,4%浓缩胶。 2、上样量40ug-50ug。